Abstract：
Canine-transmitted worms and an uncontrolled deworming program of stray dogs have caused that accurate diagnosis of zoonotic parasites received notable attention in endemic regions. This study aimed to assess the presence of E. multilocularis and E. granulosus infections in canids from Guilan province, northern Iran. A total of 272 fecal samples from carnivores were collected across 24 different regions in Guilan province between 2023 and 2024. All fecal specimens were observed following concentration and flotation techniques. DNAs of taeniid eggs were extracted, amplified, and sequenced targeting of specific mitochondrial Cox1 gene for E. granulosus and NAD 1 gene for E. multilocularis. On the base of molecular and phylogenetic analysis 1.47 % (in jackal) and 25 % (in dogs and jackal) of samples were positive for E. multilocularis and E. granulosus sensu strico G1 genotype, respectively. Molecular technique was found to be more sensitive in detecting infection in comparison with conventional techniques. Sequence analysis of Cox1 indicated a high genetic diversity (Haplotype diversity; 0.933; Number of haplotypes, h: 7) in E. granulosus G1. Current findings show that canids particularly jackals play potential role of definitive host in maintenance and transmission dynamic of E. multilocularis and E. granulosus in northern Iran. The presence of these infections is of particular concern in Guilan province due to the high influx of tourists, increasing the risk of transmission to humans. Therefore, the implementation of preventive programs is warranted to apply hygienic practices and adjusting deworming programs for the canids and at-risk individuals in the region.
摘要：
犬传播的蠕虫和流浪狗不受控制的驱虫程序已导致人畜共患寄生虫的准确诊断在流行地区引起了人们的关注。这项研究的目的是评估多房性大肠杆菌和颗粒性大肠杆菌感染的存在，伊朗北部。在2023年至2024年之间，在桂兰省的24个不同地区共收集了272个食肉动物的粪便样本。按照浓缩和浮选技术观察所有粪便标本。提取了taeniid卵的DNA，放大，并对细粒大肠杆菌的特异性线粒体Cox1基因和多房大肠杆菌的NAD1基因进行测序。在分子和系统发育分析的基础上，1.47％（在jack狼中）和25％（在狗和jack狼中）的样品对多房性大肠杆菌和GranulosussensuerricoG1基因型呈阳性，分别。与常规技术相比，发现分子技术在检测感染方面更敏感。Cox1的序列分析表明具有较高的遗传多样性（单倍型多样性；0.933；单倍型数量，h:7)在细粒大肠杆菌G1中。目前的研究结果表明，犬科动物，尤其是jack狼在伊朗北部多房大肠杆菌和细粒大肠杆菌的维持和传播动态中起着确定宿主的潜在作用。由于游客大量涌入，这些感染的存在在纪兰省尤其令人担忧，增加了传播给人类的风险。因此,有必要实施预防计划，以应用卫生习惯并调整该地区犬科动物和高危人群的驱虫计划。
