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Abstract：
Pogostemon cablin (PC) is a traditional Chinese medicine (TCM) and food as well as an important essential oil plant in China. PC essential oil exerts pharmacological effects such as anti-inflammatory, anti-oxidant, anti-platelet, anti-thrombotic, and anti-depressant. This study established a reliable and sensitive gas chromatography-mass spectrometry (GC-MS) method for the simultaneous determination of the pharmacokinetics of patchouli alcohol, β-elemene, β-caryophyllene, caryophyllene oxide, and farnesol in the plasma of rats after oral administration of PC essential oil extract. Using ethyl acetate to prepare the plasma samples, and p-menthone was used as the internal standard (IS). An HP5-MS column (0.25 μm × 0.25 mm × 30 m) was used for chromatographic separation, and detection was performed in selected ion monitoring (SIM) mode. The accuracies of intra-day and inter-day for all analytes displayed a range of -6.7 %-9.2 %, with precision below 12.5 %. Extraction recoveries for analytes ranged from 74.0 to 106.4 % and matrix effects ranged from 92.4 to 106.9 %. Stability results have demonstrated that the relative standard deviations (RSD) of analytes were below 12.1 %. Therefore, the developed GC-MS method successfully evaluated the pharmacokinetics of five volatile components in PC essential oil extract administered orally to rats.
摘要：
Pogostemoncablin（PC）是一种传统的中药（TCM）和食品，也是中国重要的精油植物。PC精油发挥药理作用，如抗炎，抗氧化剂，抗血小板,抗血栓,和抗抑郁药。本研究建立了一种可靠、灵敏的气相色谱-质谱(GC-MS)同时测定广藿香醇,β-榄香烯,β-石竹烯,石竹烯氧化物，口服PC精油提取物后，大鼠血浆中的法尼醇。用乙酸乙酯制备血浆样品,和p-薄荷酮用作内标(IS)。使用HP5-MS色谱柱（0.25μm×0.25mm×30m）进行色谱分离，在选择离子监测(SIM)模式下进行检测。所有分析物的日内和日内的准确度显示范围为-6.7％-9.2％，精度低于12.5%。分析物的提取回收率为74.0％至106.4％，基体效应为92.4％至106.9％。稳定性结果表明，分析物的相对标准偏差（RSD）低于12.1％。因此,建立的GC-MS方法成功地评估了大鼠口服PC精油提取物中5种挥发性成分的药代动力学。
