PDF(Pubmed)
Abstract：
UNASSIGNED: To analyze the antibiotic resistance profile, virulence genes, and molecular typing of Staphylococcus aureus (S. aureus) strains isolated in skin and soft tissue infections at the First Affiliated Hospital, Gannan Medical University, to better understand the molecular epidemiological characteristics of S. aureus.
UNASSIGNED: In 2023, 65 S. aureus strains were isolated from patients with skin and soft tissue infections. Strain identification and susceptibility tests were performed using VITEK 2 and gram-positive bacteria identification cards. DNA was extracted using a DNA extraction kit, and all genes were amplified using polymerase chain reaction. Multilocus sequence typing (MLST) was used for molecular typing.
UNASSIGNED: In this study, of the 65 S. aureus strains were tested for their susceptibility to 16 antibiotics, the highest resistance rate to penicillin G was 95.4%. None of the staphylococcal isolates showed resistance to ceftaroline, daptomycin, linezolid, tigecycline, teicoplanin, or vancomycin. fnbA was the most prevalent virulence gene (100%) in S. aureus strains isolated in skin and soft tissue infections, followed by arcA (98.5%). Statistical analyses showed that the resistance rates of methicillin-resistant S. aureus isolates to various antibiotics were significantly higher than those of methicillin-susceptible S. aureus isolates. Fifty sequence types (STs), including 44 new ones, were identified by MLST.
UNASSIGNED: In this study, the high resistance rate to penicillin G and the high carrying rate of virulence gene fnbA and arcA of S.aureus were determine, and 44 new STs were identified, which may be associated with the geographical location of southern Jiangxi and local trends in antibiotic use. The study of the clonal lineage and evolutionary relationships of S. aureus in these regions may help in understanding the molecular epidemiology and provide the experimental basis for pathogenic bacteria prevention and treatment.
UNASSIGNED: In 2023, 65 S. aureus strains were isolated from patients with skin and soft tissue infections. Strain identification and susceptibility tests were performed using VITEK 2 and gram-positive bacteria identification cards. DNA was extracted using a DNA extraction kit, and all genes were amplified using polymerase chain reaction. Multilocus sequence typing (MLST) was used for molecular typing.
UNASSIGNED: In this study, of the 65 S. aureus strains were tested for their susceptibility to 16 antibiotics, the highest resistance rate to penicillin G was 95.4%. None of the staphylococcal isolates showed resistance to ceftaroline, daptomycin, linezolid, tigecycline, teicoplanin, or vancomycin. fnbA was the most prevalent virulence gene (100%) in S. aureus strains isolated in skin and soft tissue infections, followed by arcA (98.5%). Statistical analyses showed that the resistance rates of methicillin-resistant S. aureus isolates to various antibiotics were significantly higher than those of methicillin-susceptible S. aureus isolates. Fifty sequence types (STs), including 44 new ones, were identified by MLST.
UNASSIGNED: In this study, the high resistance rate to penicillin G and the high carrying rate of virulence gene fnbA and arcA of S.aureus were determine, and 44 new STs were identified, which may be associated with the geographical location of southern Jiangxi and local trends in antibiotic use. The study of the clonal lineage and evolutionary relationships of S. aureus in these regions may help in understanding the molecular epidemiology and provide the experimental basis for pathogenic bacteria prevention and treatment.
摘要：
■为了分析抗生素耐药性，毒力基因，和金黄色葡萄球菌的分子分型(S.金黄色葡萄球菌）在第一附属医院皮肤和软组织感染中分离出的菌株，赣南医科大学,为了更好地了解金黄色葡萄球菌的分子流行病学特征。
■2023年，从皮肤和软组织感染的患者中分离出65株金黄色葡萄球菌菌株。使用VITEK2和革兰氏阳性菌鉴定卡进行菌株鉴定和药敏试验。用DNA提取试剂盒提取DNA,所有基因都用聚合酶链反应扩增。多位点序列分型(MLST)用于分子分型。
■在这项研究中，对65株金黄色葡萄球菌菌株进行了对16种抗生素的敏感性测试，青霉素G耐药率最高,达95.4%。葡萄球菌分离株均未显示对头孢洛林的耐药性，达托霉素,利奈唑胺,替加环素,替考拉宁,或者万古霉素.fnbA是皮肤和软组织感染中分离的金黄色葡萄球菌菌株中最普遍的毒力基因(100%)，其次是arcA(98.5%)。统计分析表明，耐甲氧西林金黄色葡萄球菌对各种抗生素的耐药率明显高于耐甲氧西林金黄色葡萄球菌。五十个序列类型(STs)，包括44个新的，由MLST鉴定。
■在这项研究中，确定了金黄色葡萄球菌对青霉素G的高耐药率以及毒力基因fnbA和arcA的高携带率，并确定了44个新的STs，这可能与江西南部的地理位置和当地抗生素使用趋势有关。研究这些地区金黄色葡萄球菌的克隆谱系和进化关系有助于理解其分子流行病学，为病原菌的预防和治疗提供实验依据。
■2023年，从皮肤和软组织感染的患者中分离出65株金黄色葡萄球菌菌株。使用VITEK2和革兰氏阳性菌鉴定卡进行菌株鉴定和药敏试验。用DNA提取试剂盒提取DNA,所有基因都用聚合酶链反应扩增。多位点序列分型(MLST)用于分子分型。
■在这项研究中，对65株金黄色葡萄球菌菌株进行了对16种抗生素的敏感性测试，青霉素G耐药率最高,达95.4%。葡萄球菌分离株均未显示对头孢洛林的耐药性，达托霉素,利奈唑胺,替加环素,替考拉宁,或者万古霉素.fnbA是皮肤和软组织感染中分离的金黄色葡萄球菌菌株中最普遍的毒力基因(100%)，其次是arcA(98.5%)。统计分析表明，耐甲氧西林金黄色葡萄球菌对各种抗生素的耐药率明显高于耐甲氧西林金黄色葡萄球菌。五十个序列类型(STs)，包括44个新的，由MLST鉴定。
■在这项研究中，确定了金黄色葡萄球菌对青霉素G的高耐药率以及毒力基因fnbA和arcA的高携带率，并确定了44个新的STs，这可能与江西南部的地理位置和当地抗生素使用趋势有关。研究这些地区金黄色葡萄球菌的克隆谱系和进化关系有助于理解其分子流行病学，为病原菌的预防和治疗提供实验依据。
