PDF(Pubmed)
Abstract：
UNASSIGNED: Antimicrobial resistance is increasingly becoming a global health concern. This study aimed to investigate and report MDR Escherichia coli (E. coli) prevalence, resistance, and virulence genes from poultry in Jos, Plateau State, Nigeria.
UNASSIGNED: The samples were analyzed using microbiological standard methods and polymerase chain reactions (PCRs).
UNASSIGNED: A total of 179 cloacal swabs were collected from bothlocal and exotic poultry breeds, of which 99.4% (178/179) tested positive for E. coli. Among these culturally identified samples, 99.4% (177/178) were furtherconfirmed Escherichia coli with a molecular weight of 401 bp. Multidrugresistance of 45% (80/178) was observed from the confirmed isolates. PCR assays were conducted to detect genes associated with resistance to antibiotics, specifically, tetracycline (tetA gene), sulfonamide (sul1 gene), ampicillin (ampC gene), and quinolone (gyrA gene). Antimicrobial susceptibility test (AST) results revealed substantial antibiotic resistance, with 81.9% (145/177) of the isolates being resistant to tetracycline, 80.2% (142/177) to quinolone, 69.5% (123/177) to sulfonamide, and 66.1% (117/177) to ampicillin. Further analysis on 18 isolates that showed resistance to up to four different antibiotics was carried out using multiplex PCR to detect eae, hlyA, rfbE, fliC, and fstx virulence genes. The study found that 44.4% (15/18) of the isolates were positive for the eae gene, 27.7% (5/18) for stx, 22.2% (4/18) for rfbe gene, and 5.5% (1) for hlya gene, and none tested positive for fliC gene.
UNASSIGNED: These results showed high antibiotic resistance, virulent genes, and significant levels of MDR in E. coli from poultry. This study highlights the urgent need for antimicrobial stewardship practices within the poultry industry due to their profound implications for food safety and public health. This issue is particularly critical in Nigeria, where poultry farming constitutes a significant portion of smallholder farming practices.
UNASSIGNED: The samples were analyzed using microbiological standard methods and polymerase chain reactions (PCRs).
UNASSIGNED: A total of 179 cloacal swabs were collected from bothlocal and exotic poultry breeds, of which 99.4% (178/179) tested positive for E. coli. Among these culturally identified samples, 99.4% (177/178) were furtherconfirmed Escherichia coli with a molecular weight of 401 bp. Multidrugresistance of 45% (80/178) was observed from the confirmed isolates. PCR assays were conducted to detect genes associated with resistance to antibiotics, specifically, tetracycline (tetA gene), sulfonamide (sul1 gene), ampicillin (ampC gene), and quinolone (gyrA gene). Antimicrobial susceptibility test (AST) results revealed substantial antibiotic resistance, with 81.9% (145/177) of the isolates being resistant to tetracycline, 80.2% (142/177) to quinolone, 69.5% (123/177) to sulfonamide, and 66.1% (117/177) to ampicillin. Further analysis on 18 isolates that showed resistance to up to four different antibiotics was carried out using multiplex PCR to detect eae, hlyA, rfbE, fliC, and fstx virulence genes. The study found that 44.4% (15/18) of the isolates were positive for the eae gene, 27.7% (5/18) for stx, 22.2% (4/18) for rfbe gene, and 5.5% (1) for hlya gene, and none tested positive for fliC gene.
UNASSIGNED: These results showed high antibiotic resistance, virulent genes, and significant levels of MDR in E. coli from poultry. This study highlights the urgent need for antimicrobial stewardship practices within the poultry industry due to their profound implications for food safety and public health. This issue is particularly critical in Nigeria, where poultry farming constitutes a significant portion of smallholder farming practices.
摘要：
抗菌素耐药性正日益成为全球健康问题。本研究旨在调查和报道MDR大肠杆菌(E.大肠杆菌)患病率，阻力,和乔斯家禽的毒力基因，高原州,尼日利亚。
■使用微生物标准方法和聚合酶链反应（PCR）分析样品。
■从本地和外来家禽品种中总共收集了179个泄殖腔拭子，其中99.4%(178/179)的大肠杆菌检测呈阳性。在这些文化上确定的样本中，99.4%(177/178)是进一步证实的分子量为401bp的大肠杆菌。从确认的分离物中观察到45％（80/178）的多药物抗性。进行PCR测定以检测与抗生素抗性相关的基因，具体来说,四环素（tetA基因），磺酰胺（sul1基因），氨苄青霉素（ampC基因），和喹诺酮（gyrA基因）。抗菌药物敏感性试验(AST)结果显示有相当大的抗生素耐药性,81.9%(145/177)的菌株对四环素耐药，80.2%(142/177)对喹诺酮,69.5%(123/177)对磺酰胺，氨苄青霉素占66.1%(117/177)。使用多重PCR检测eae，对18株对多达4种不同抗生素具有抗性的分离株进行了进一步分析，hlyA,rfbE,FILC,和fstx毒力基因.研究发现,44.4%(15/18)的分离株eae基因阳性,stx为27.7%（5/18），22.2%(4/18)为rfbe基因，和5.5％（1）的hlya基因，而且没有一个基因检测呈阳性.
■这些结果显示高抗生素耐药性，毒力基因,以及家禽大肠杆菌中显著水平的MDR。这项研究强调了在家禽业中迫切需要抗菌管理实践，因为它们对食品安全和公共卫生具有深远的影响。这个问题在尼日利亚尤为关键，家禽养殖构成小农养殖实践的重要部分。
■使用微生物标准方法和聚合酶链反应（PCR）分析样品。
■从本地和外来家禽品种中总共收集了179个泄殖腔拭子，其中99.4%(178/179)的大肠杆菌检测呈阳性。在这些文化上确定的样本中，99.4%(177/178)是进一步证实的分子量为401bp的大肠杆菌。从确认的分离物中观察到45％（80/178）的多药物抗性。进行PCR测定以检测与抗生素抗性相关的基因，具体来说,四环素（tetA基因），磺酰胺（sul1基因），氨苄青霉素（ampC基因），和喹诺酮（gyrA基因）。抗菌药物敏感性试验(AST)结果显示有相当大的抗生素耐药性,81.9%(145/177)的菌株对四环素耐药，80.2%(142/177)对喹诺酮,69.5%(123/177)对磺酰胺，氨苄青霉素占66.1%(117/177)。使用多重PCR检测eae，对18株对多达4种不同抗生素具有抗性的分离株进行了进一步分析，hlyA,rfbE,FILC,和fstx毒力基因.研究发现,44.4%(15/18)的分离株eae基因阳性,stx为27.7%（5/18），22.2%(4/18)为rfbe基因，和5.5％（1）的hlya基因，而且没有一个基因检测呈阳性.
■这些结果显示高抗生素耐药性，毒力基因,以及家禽大肠杆菌中显著水平的MDR。这项研究强调了在家禽业中迫切需要抗菌管理实践，因为它们对食品安全和公共卫生具有深远的影响。这个问题在尼日利亚尤为关键，家禽养殖构成小农养殖实践的重要部分。
