PDF(Pubmed)
Abstract：
UNASSIGNED: MicroRNAs (miRNAs, miRs) have been linked to beta-cell pathologies and have also shown potential as biomarkers for cardiovascular disease. This study aimed to evaluate the expression of miR-375 and miR-541 in T2D patients with and without CAD, in order to determine the potential of these miRNAs as biomarkers for assessing CAD risk.
UNASSIGNED: This study was conducted on 106 patients with T2D who underwent coronary angiographic examination. Reverse transcription was performed using the cDNA synthesis kit. Real-time PCR was performed using the SYBR Green method and specific primers. The ability to predict which person had developed CAD was evaluated by calculating the area under the receiver-operating characteristic (ROC) curve (AUC).
UNASSIGNED: The expression of miR-375 was significantly higher in samples from CAD patients compared to those without CAD (p = 0.009). While the expression of miR-541 was also higher in CAD patients, the difference was not statistically significant. In terms of predicting CAD, miR-375 was found to be a suitable predictor with an AUC of 0.74 (p = 0.01), while miR-541 was not. With a cut-off value of 0.016 for miR-375, the sensitivity was 67% and the specificity was 80%.
UNASSIGNED: Our results indicated that circulating levels of miR-375 and miR-541 were elevated in T2D patients with CAD compared to those without CAD. This suggests that miR-375 could potentially be used as a non-invasive biomarker for the diagnosis of CAD in T2D patients.
UNASSIGNED: This study was conducted on 106 patients with T2D who underwent coronary angiographic examination. Reverse transcription was performed using the cDNA synthesis kit. Real-time PCR was performed using the SYBR Green method and specific primers. The ability to predict which person had developed CAD was evaluated by calculating the area under the receiver-operating characteristic (ROC) curve (AUC).
UNASSIGNED: The expression of miR-375 was significantly higher in samples from CAD patients compared to those without CAD (p = 0.009). While the expression of miR-541 was also higher in CAD patients, the difference was not statistically significant. In terms of predicting CAD, miR-375 was found to be a suitable predictor with an AUC of 0.74 (p = 0.01), while miR-541 was not. With a cut-off value of 0.016 for miR-375, the sensitivity was 67% and the specificity was 80%.
UNASSIGNED: Our results indicated that circulating levels of miR-375 and miR-541 were elevated in T2D patients with CAD compared to those without CAD. This suggests that miR-375 could potentially be used as a non-invasive biomarker for the diagnosis of CAD in T2D patients.
摘要：
■微RNA（miRNA，miRs）已与β细胞病理有关，并且还显示出作为心血管疾病生物标志物的潜力。本研究旨在评估miR-375和miR-541在伴有和不伴有CAD的T2D患者中的表达。以确定这些miRNA作为评估CAD风险的生物标志物的潜力。
■本研究对106例T2D患者进行了冠状动脉造影检查。使用cDNA合成试剂盒进行逆转录。使用SYBRGreen方法和特异性引物进行实时PCR。通过计算受试者工作特征（ROC）曲线（AUC）下的面积来评估预测哪个人患有CAD的能力。
■来自CAD患者的样本中miR-375的表达显著高于没有CAD的那些(p=0.009)。虽然miR-541在CAD患者中的表达也较高，差异无统计学意义。在预测CAD方面，miR-375被发现是一个合适的预测因子，AUC为0.74（p=0.01），而miR-541则不是。miR-375的截断值为0.016，敏感性为67%，特异性为80%。
■我们的结果表明，与没有CAD的患者相比，在患有CAD的T2D患者中miR-375和miR-541的循环水平升高。这表明miR-375可能用作诊断T2D患者CAD的非侵入性生物标志物。
■本研究对106例T2D患者进行了冠状动脉造影检查。使用cDNA合成试剂盒进行逆转录。使用SYBRGreen方法和特异性引物进行实时PCR。通过计算受试者工作特征（ROC）曲线（AUC）下的面积来评估预测哪个人患有CAD的能力。
■来自CAD患者的样本中miR-375的表达显著高于没有CAD的那些(p=0.009)。虽然miR-541在CAD患者中的表达也较高，差异无统计学意义。在预测CAD方面，miR-375被发现是一个合适的预测因子，AUC为0.74（p=0.01），而miR-541则不是。miR-375的截断值为0.016，敏感性为67%，特异性为80%。
■我们的结果表明，与没有CAD的患者相比，在患有CAD的T2D患者中miR-375和miR-541的循环水平升高。这表明miR-375可能用作诊断T2D患者CAD的非侵入性生物标志物。
