PDF(Pubmed)
Abstract：
UNASSIGNED: Obesity has been linked to a higher risk of postmenopausal breast cancer Yet, research indicates an opposite correlation between obesity and premenopausal breast cancer risk. Various obesity phenotypes based on metabolic health could play a significant part. This study aims to assess how plasma exosomes taken from women with varying obesity phenotypes impact MCF-7 cell migration, matrix metalloproteinase-2 activity, and apoptosis.
UNASSIGNED: The characterization of isolated exosomes and their internalization into MCF-7 cells was evaluated. The treatment of MCF-7 cells with exosomes isolated from different groups was done. Migration, the activity of MMP-2, mRNA expression of Bax and Bcl-2, protein expression of p-53 and Thr55 p-p53, and apoptosis were assessed.
UNASSIGNED: Isolated exosomes from unhealthy obese individuals increase MCF-7 cell migration. Regarding MMP activities, unhealthy normal weight and overweight and healthy obese groups isolated exosomes increase the MMP-2 activity than the treated group with exosomes isolated from counterpart groups. Furthermore, unhealthy normal weight and overweight and healthy obese obtained exosomes decrease apoptosis compared to counterpart groups.
UNASSIGNED: Altogether, plasma exosomes derived from both unhealthy individuals with normal weight and overweight status, as well as those with unhealthy obesity, negatively impacted the behavior of estrogen/progesterone receptor-positive breast cancer cells.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s40200-023-01295-1.
UNASSIGNED: The characterization of isolated exosomes and their internalization into MCF-7 cells was evaluated. The treatment of MCF-7 cells with exosomes isolated from different groups was done. Migration, the activity of MMP-2, mRNA expression of Bax and Bcl-2, protein expression of p-53 and Thr55 p-p53, and apoptosis were assessed.
UNASSIGNED: Isolated exosomes from unhealthy obese individuals increase MCF-7 cell migration. Regarding MMP activities, unhealthy normal weight and overweight and healthy obese groups isolated exosomes increase the MMP-2 activity than the treated group with exosomes isolated from counterpart groups. Furthermore, unhealthy normal weight and overweight and healthy obese obtained exosomes decrease apoptosis compared to counterpart groups.
UNASSIGNED: Altogether, plasma exosomes derived from both unhealthy individuals with normal weight and overweight status, as well as those with unhealthy obesity, negatively impacted the behavior of estrogen/progesterone receptor-positive breast cancer cells.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s40200-023-01295-1.
摘要：
■肥胖与绝经后乳腺癌的高风险有关。研究表明，肥胖与绝经前乳腺癌风险之间存在相反的相关性。基于代谢健康的各种肥胖表型可能发挥重要作用。本研究旨在评估来自不同肥胖表型女性的血浆外泌体如何影响MCF-7细胞迁移。基质金属蛋白酶-2活性，和凋亡。
■评估分离的外来体的表征及其内化到MCF-7细胞中。用分离自不同组的外泌体处理MCF-7细胞。迁移,观察MMP-2活性、Bax和Bcl-2mRNA表达、p-53和Thr55p-p53蛋白表达及细胞凋亡情况。
■从不健康肥胖个体中分离的外泌体增加MCF-7细胞迁移。关于MMP活动，不健康的正常体重和超重以及健康肥胖组分离的外泌体比治疗组的外泌体增加MMP-2活性。此外，与对应组相比,不健康的正常体重和超重以及健康肥胖获得的外泌体减少了细胞凋亡.
■总之，来自正常体重和超重状态的不健康个体的血浆外泌体，以及那些不健康肥胖的人，对雌激素/孕激素受体阳性乳腺癌细胞的行为产生负面影响。
■在线版本包含补充材料，可在10.1007/s40200-023-01295-1获得。
■评估分离的外来体的表征及其内化到MCF-7细胞中。用分离自不同组的外泌体处理MCF-7细胞。迁移,观察MMP-2活性、Bax和Bcl-2mRNA表达、p-53和Thr55p-p53蛋白表达及细胞凋亡情况。
■从不健康肥胖个体中分离的外泌体增加MCF-7细胞迁移。关于MMP活动，不健康的正常体重和超重以及健康肥胖组分离的外泌体比治疗组的外泌体增加MMP-2活性。此外，与对应组相比,不健康的正常体重和超重以及健康肥胖获得的外泌体减少了细胞凋亡.
■总之，来自正常体重和超重状态的不健康个体的血浆外泌体，以及那些不健康肥胖的人，对雌激素/孕激素受体阳性乳腺癌细胞的行为产生负面影响。
■在线版本包含补充材料，可在10.1007/s40200-023-01295-1获得。
