%0 Journal Article
%T Exosomes isolated from metabolically unhealthy normal weight and overweight phenotypes deteriorate the ER/PR positive breast cancer behavior.
%A Esmaeili F
%A Abolhasani M
%A Zabihi-Mahmoudabadi H
%A Seyyed Ebrahimi SS
%A Emamgholipour S
%A Paknejad M
%J J Diabetes Metab Disord
%V 23
%N 1
%D 2024 Jun
%M 38932828
暂无%R 10.1007/s40200-023-01295-1
%X <B>UNASSIGNED: </B>Obesity has been linked to a higher risk of postmenopausal breast cancer Yet, research indicates an opposite correlation between obesity and premenopausal breast cancer risk. Various obesity phenotypes based on metabolic health could play a significant part. This study aims to assess how plasma exosomes taken from women with varying obesity phenotypes impact MCF-7 cell migration, matrix metalloproteinase-2 activity, and apoptosis.<BR><B>UNASSIGNED: </B>The characterization of isolated exosomes and their internalization into MCF-7 cells was evaluated. The treatment of MCF-7 cells with exosomes isolated from different groups was done. Migration, the activity of MMP-2, mRNA expression of Bax and Bcl-2, protein expression of p-53 and Thr55 p-p53, and apoptosis were assessed.<BR><B>UNASSIGNED: </B>Isolated exosomes from unhealthy obese individuals increase MCF-7 cell migration. Regarding MMP activities, unhealthy normal weight and overweight and healthy obese groups isolated exosomes increase the MMP-2 activity than the treated group with exosomes isolated from counterpart groups. Furthermore, unhealthy normal weight and overweight and healthy obese obtained exosomes decrease apoptosis compared to counterpart groups.<BR><B>UNASSIGNED: </B>Altogether, plasma exosomes derived from both unhealthy individuals with normal weight and overweight status, as well as those with unhealthy obesity, negatively impacted the behavior of estrogen/progesterone receptor-positive breast cancer cells.<BR><B>UNASSIGNED: </B>The online version contains supplementary material available at 10.1007/s40200-023-01295-1.