PDF(Pubmed)
Abstract：
The serological surveillance of bluetongue in bulk tank milk is an efficient and cost-effective method for the early detection of bluetongue virus incursions in unvaccinated free areas of the disease. In addition, the availability of standardized and reliable reagents and refined diagnostic procedures with high sensitivity and specificity are essential for surveillance purposes. However, no available reference materials for bluetongue virus serological surveillance in bulk tank milk exist. This study shows the production and characterization of reference material for the implementation of a commercially available bluetongue milk ELISA test in official laboratories, as well as the evaluation of a procedure to increase the sensitivity in samples with low levels of antibodies. This procedure, based on milk protein concentration, allowed us to notably increase the ELISA test\'s analytical sensitivity, which is useful for milk samples from farms with low within-herd prevalence or pools of bulk tank milk samples. The standardized milk reference material produced here, together with the evaluated procedure to improve analytical sensitivity, could be applied as tools to ensure an accurate diagnosis by official laboratories in bluetongue unvaccinated free areas.
摘要：
散装罐乳中蓝舌病的血清学监测是一种有效且具有成本效益的方法，可在未接种疫苗的游离地区早期检测蓝舌病病毒的入侵。此外,标准化和可靠的试剂的可用性以及具有高灵敏度和特异性的完善的诊断程序对于监测目的至关重要.然而,没有可用的散装罐奶蓝舌病毒血清学监测参考材料。这项研究显示了在官方实验室中实施市售蓝舌乳ELISA测试的参考材料的生产和表征，以及评估增加抗体水平低的样品灵敏度的程序。这个程序,基于牛奶蛋白质浓度，使我们能够显著提高ELISA测试的分析灵敏度，这对来自牛群内流行率低的农场的牛奶样品或散装罐牛奶样品池很有用。这里生产的标准化牛奶参考材料，以及提高分析灵敏度的评估程序，可以作为工具，以确保在蓝舌未接种疫苗的免费地区的官方实验室进行准确的诊断。
