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Abstract：
Glycerophospholipids have hydrophobic and hydrophilic moieties. Previous studies suggest that phospholipids with different moieties have different effects on rodent behavior; however, the relationship between chemical structures and behavioral effects remains unclear. To clarify the functions of phospholipid moieties, we injected male rats with phospholipids with different moieties and conducted behavioral tests. Exploratory activity was reduced by phosphatidylethanolamine (PE)(18:0/22:6) but not PE(18:0/18:0) or PE(18:0/20:4). Conversely, exploratory activity was increased by plasmanyl PE(16:0/22:6), which harbors an alkyl-ether linkage, but not by phosphatidylcholine (PC)(16:0/22:6) or plasmanyl PC(16:0/22:6). Docosahexaenoic acid (DHA)(22:6) and an alkyl-ether linkage in PE were thus postulated to be involved in exploratory activity. Anxiety-like behavior was reduced by plasmenyl PC(18:0/20:4), which harbors a vinyl-ether linkage, but not by PC(18:0/20:4) or plasmanyl PC(18:0/20:4), suggesting the anxiolytic effects of vinyl-ether linkage. The activation of social interaction was suppressed by PE(18:0/18:0), PE(18:0/22:6), PC(16:0/22:6), plasmanyl PE(16:0/22:6), and plasmanyl PC(16:0/22:6) but not by PE(18:0/20:4), plasmenyl PE(18:0/20:4), or plasmanyl PC(18:0/22:6). DHA may suppress social interaction, whereas arachidonic acid(20:4) or a combination of alkyl-ether linkage and stearic acid(18:0) may restore social deficits. Our findings indicate the characteristic effects of different phospholipid moieties on rat behavior, and may help to elucidate patterns between chemical structures and their effects.
摘要：
甘油磷脂具有疏水和亲水部分。以前的研究表明，不同部分的磷脂对啮齿动物的行为有不同的影响;然而，化学结构与行为效应之间的关系尚不清楚.为了阐明磷脂部分的功能，我们向雄性大鼠注射不同部分的磷脂，并进行行为测试。磷脂酰乙醇胺（PE）（18：0/22：6）降低了探索性活性，但PE（18：0/18：0）或PE（18：0/20：4）没有降低。相反，血浆丙氨酰PE(16:0/22:6)增加了探索性活性，带有烷基-醚键，但不是通过磷脂酰胆碱（PC）（16：0/22：6）或血浆酰PC（16：0/22：6）。因此，假定二十二碳六烯酸（DHA）（22：6）和PE中的烷基-醚键参与了探索活动。类焦虑行为减少了plasmenylPC（18：0/20：4），带有乙烯基醚键，但不是由PC（18：0/20：4）或plasmanylPC（18：0/20：4），提示了乙烯基醚键的抗焦虑作用。社会交往的激活受到PE(18:0/18:0)的抑制，PE(18:0/22:6)，PC(16:0/22:6),plasmanylPE(16:0/22:6)，和plasmanylPC（16：0/22：6），但不是PE（18：0/20：4），plasmenylPE（18:0/20:4），或plasmanylPC（18：0/22：6）。DHA可能会抑制社交互动，而花生四烯酸（20：4）或烷基醚键和硬脂酸（18：0）的组合可以恢复社会赤字。我们的发现表明不同磷脂部分对大鼠行为的特征性影响，并可能有助于阐明化学结构及其作用之间的模式。
