PDF(Pubmed)
Abstract：
Skin lesions are an important health concern, exposing the body to infection risks. Utilizing natural products containing chamomile (Chamomilla recutita L.) holds promise for curative purposes. Additionally, hyaluronic acid (HA), an active ingredient known for its tissue regeneration capacity, can expedite healing. In this study, we prepared and characterized an extract of C. recutita and integrated it into a nanoemulsion system stabilized with HA, aiming at harnessing its healing potential. We assessed the impact of alcoholic strength on flavonoid extraction and chemically characterized the extract using UHPLC/MS while quantifying its antioxidant and antimicrobial capacity. We developed a nanoemulsion loaded with C. recutita extract and evaluated the effect of HA stabilization on pH, droplet size, polydispersity index (PDI), zeta potential, and viscosity. Results indicated that 70% hydroalcoholic extraction yielded a higher flavonoid content. The extract exhibited antioxidant capacity in vitro, a desirable trait for skin regeneration, and demonstrated efficacy against key microbial strains (Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa) associated with skin colonization and infections. Flavonoids spireoside and apiin emerged as the most abundant bioactives. The addition of HA led to increased viscosity while maintaining a suitable pH for topical application. Zeta potential, droplet size, and PDI met acceptable criteria. Moreover, incorporating C. recutita extract into the nanoemulsion enhanced its antimicrobial effect. Hence, the nanoemulsion system loaded with C. recutita and HA stabilization exhibits favorable characteristics for topical application, showing promise in aiding the healing processes.
摘要：
皮肤损伤是一个重要的健康问题，将身体暴露于感染风险。利用含有洋甘菊（ChamomillarecuttaL.）的天然产品有望达到治疗目的。此外,透明质酸（HA），一种以其组织再生能力而闻名的活性成分，可以加速愈合。在这项研究中,我们制备并表征了C.recutta的提取物，并将其整合到用HA稳定的纳米乳液系统中，旨在利用其治愈潜力。我们评估了酒精强度对类黄酮提取的影响，并使用UHPLC/MS对提取物进行化学表征，同时定量其抗氧化和抗菌能力。我们开发了装载有C.recutta提取物的纳米乳液，并评估了HA稳定对pH的影响，液滴大小,多分散指数(PDI)，zeta电位,和粘度。结果表明，70％水醇提取产生较高的类黄酮含量。提取物在体外表现出抗氧化能力，皮肤再生的理想特征，并证明了对关键微生物菌株(金黄色葡萄球菌，化脓性链球菌,大肠杆菌,和铜绿假单胞菌）与皮肤定植和感染有关。黄酮类螺旋体苷和芹菜苷是最丰富的生物活性物质。HA的添加导致粘度增加,同时保持适合局部施用的pH。Zeta电位,液滴大小,和PDI符合可接受的标准。此外，将C.recutta提取物掺入纳米乳液中增强了其抗菌作用。因此，负载有C.recutta和HA稳定的纳米乳液系统表现出局部应用的有利特性，显示出帮助愈合过程的希望。
