PDF(Pubmed)
Abstract：
To monitor the biological function of H2S in real time, this investigation demonstrated the design and synthesis of a novel fluorescent probe integrated with cyanine and 2,4-dinitrophenol for the qualitative and quantitative detection of H2S. An NIR sensitive sensor (FS-HS-1) was provided with a straightforward process. Spectroscopy experiments elucidated that FS-HS-1 could selectively detect H2S in a PBS solution (containing 40% acetonitrile) with a 111-fold fluorescence enhancement at 715 nm (ex. 605 nm). The response towards NaHS occurred in less than 2 min, and the detection limit was confirmed to be as low as 4.47 ± 0.11 nmol/L. Furthermore, the probe is capable of monitoring changes in exogenous H2S concentrations within living cells with confocal and 2P imaging.
摘要：
实时监测H2S的生物功能,这项研究证明了与花青和2,4-二硝基苯酚集成的新型荧光探针的设计和合成，用于定性和定量检测H2S。NIR敏感传感器(FS-HS-1)具有简单的过程。光谱实验表明，FS-HS-1可以选择性检测PBS溶液（含40％乙腈）中的H2S，在715nm处荧光增强111倍（例如605nm）。对NaHS的反应发生在不到2分钟内，检测限低至4.47±0.11nmol/L。此外，该探针能够通过共聚焦和2P成像监测活细胞内外源性H2S浓度的变化。
