{Reference Type}: Journal Article
{Title}: Practical NIR Assay Derived from Cyanine to Evaluate Intracellular H2S in Living Cell Imaging.
{Author}: Ye C;Wang A;Lu Y;Lin X;Huang L;Li D;
{Journal}: Sensors (Basel)
{Volume}: 24
{Issue}: 12
{Year}: 2024 Jun 8
{Factor}: 3.847
{DOI}: 10.3390/s24123744
{Abstract}: To monitor the biological function of H2S in real time, this investigation demonstrated the design and synthesis of a novel fluorescent probe integrated with cyanine and 2,4-dinitrophenol for the qualitative and quantitative detection of H2S. An NIR sensitive sensor (FS-HS-1) was provided with a straightforward process. Spectroscopy experiments elucidated that FS-HS-1 could selectively detect H2S in a PBS solution (containing 40% acetonitrile) with a 111-fold fluorescence enhancement at 715 nm (ex. 605 nm). The response towards NaHS occurred in less than 2 min, and the detection limit was confirmed to be as low as 4.47 ± 0.11 nmol/L. Furthermore, the probe is capable of monitoring changes in exogenous H2S concentrations within living cells with confocal and 2P imaging.