PDF(Pubmed)
Abstract：
BACKGROUND: Due to its rapid resistance development and ability to form biofilms, treatment of Pseudomonas aeruginosa infections is becoming more complicated by the day. Drug combinations may help reduce both resistance and biofilm formation.
METHODS: Using the microtiter plate assay, we investigated the in vitro inhibition of biofilm formation and the disruption of preformed biofilms in multidrug-resistant and extensively drug-resistant clinical isolates of P. aeruginosa in the presence of peak plasma levels of eight antipseudomonal antibiotics alone and in combination with fosfomycin: ceftazidime, piperacillin/tazobactam, cefepime, imipenem, gentamicin, amikacin, ciprofloxacin and colistin.
RESULTS: Combination therapy was significantly superior to monotherapy in its inhibition of biofilm formation. The highest inhibition rates were observed for combinations with colistin, cefepime and ceftazidime.
CONCLUSIONS: Our results support fosfomycin combination therapy as an enhanced prophylactic option. Moreover, combinations with β-lactam antibiotics and colistin demonstrated a more potent inhibition effect on biofilm formation than protein synthesis inhibitors.
METHODS: Using the microtiter plate assay, we investigated the in vitro inhibition of biofilm formation and the disruption of preformed biofilms in multidrug-resistant and extensively drug-resistant clinical isolates of P. aeruginosa in the presence of peak plasma levels of eight antipseudomonal antibiotics alone and in combination with fosfomycin: ceftazidime, piperacillin/tazobactam, cefepime, imipenem, gentamicin, amikacin, ciprofloxacin and colistin.
RESULTS: Combination therapy was significantly superior to monotherapy in its inhibition of biofilm formation. The highest inhibition rates were observed for combinations with colistin, cefepime and ceftazidime.
CONCLUSIONS: Our results support fosfomycin combination therapy as an enhanced prophylactic option. Moreover, combinations with β-lactam antibiotics and colistin demonstrated a more potent inhibition effect on biofilm formation than protein synthesis inhibitors.
摘要：
背景：由于其快速的抗性发展和形成生物膜的能力，铜绿假单胞菌感染的治疗日益复杂。药物组合可能有助于减少耐药性和生物膜形成。
方法：使用微量滴定板测定法，我们研究了在多重耐药和广泛耐药的铜绿假单胞菌临床分离株中，在存在八种抗假单胞菌的血浆峰值水平的情况下，生物膜形成的体外抑制和预制生物膜的破坏单独和与磷霉素联合使用的头孢他啶，哌拉西林/他唑巴坦,头孢吡肟,亚胺培南,庆大霉素,阿米卡星，环丙沙星和粘菌素.
结果：联合治疗在抑制生物膜形成方面明显优于单一治疗。与粘菌素的组合观察到最高的抑制率，头孢吡肟和头孢他啶.
结论：我们的结果支持磷霉素联合治疗作为一种增强的预防选择。此外，与β-内酰胺抗生素和粘菌素的组合比蛋白质合成抑制剂对生物膜形成的抑制作用更强。
方法：使用微量滴定板测定法，我们研究了在多重耐药和广泛耐药的铜绿假单胞菌临床分离株中，在存在八种抗假单胞菌的血浆峰值水平的情况下，生物膜形成的体外抑制和预制生物膜的破坏单独和与磷霉素联合使用的头孢他啶，哌拉西林/他唑巴坦,头孢吡肟,亚胺培南,庆大霉素,阿米卡星，环丙沙星和粘菌素.
结果：联合治疗在抑制生物膜形成方面明显优于单一治疗。与粘菌素的组合观察到最高的抑制率，头孢吡肟和头孢他啶.
结论：我们的结果支持磷霉素联合治疗作为一种增强的预防选择。此外，与β-内酰胺抗生素和粘菌素的组合比蛋白质合成抑制剂对生物膜形成的抑制作用更强。
