PDF(Pubmed)
Abstract：
Pearl oysters have been extensively utilized in pearl production; however, most pearl oyster shells are discarded as industrial waste. In a previous study, we demonstrated that the intraperitoneal administration of pearl oyster shell-derived nacre extract (NE) prevented d-galactose-induced brain and skin aging. In this study, we examined the anti-aging effects of orally administered NE in senescence-accelerated mice (SAMP8). Feeding SAMP8 mice NE prevented the development of aging-related characteristics, such as coarse and dull hair, which are commonly observed in aged mice. Additionally, the NE mitigated muscle aging in SAMP8 mice, such as a decline in grip strength. Histological analysis of skeletal muscle revealed that the NE suppressed the expression of aging markers, cyclin-dependent kinase inhibitor 2A (p16) and cyclin-dependent kinase inhibitor 1 (p21), and increased the expression of sirtuin1 and peroxisome proliferator-activated receptor gamma coactivator 1 (PGC1)- α, which are involved in muscle synthesis. These findings suggest that the oral administration of NE suppresses skeletal muscle aging. Moreover, NE administration suppressed skin aging, including a decline in water content. Interestingly, oral administration of NE significantly extended the lifespan of SAMP8 mice, suggesting that its effectiveness as an anti-aging agent of various tissues including skeletal muscle, skin, and adipose tissue.
摘要：
珍珠牡蛎已广泛用于珍珠生产;然而，大多数珍珠牡蛎壳被作为工业废物丢弃。在之前的研究中,我们证明了腹膜内施用珍珠牡蛎壳衍生的珍珠质提取物（NE）可防止d-半乳糖诱导的大脑和皮肤老化。在这项研究中,我们检查了口服NE对衰老加速小鼠（SAMP8）的抗衰老作用。喂养SAMP8小鼠NE阻止衰老相关特征的发展，如粗糙和沉闷的头发，通常在老年小鼠中观察到。此外,NE减轻SAMP8小鼠的肌肉老化，如握力下降。骨骼肌的组织学分析显示，NE抑制衰老标志物的表达，细胞周期蛋白依赖性激酶抑制剂2A（p16）和细胞周期蛋白依赖性激酶抑制剂1（p21），并增加sirtuin1和过氧化物酶体增殖物激活受体γ辅激活因子1（PGC1）-α的表达，参与肌肉合成。这些发现表明，口服NE可以抑制骨骼肌衰老。此外，NE给药抑制皮肤老化，包括含水量的下降。有趣的是,口服NE可显着延长SAMP8小鼠的寿命，表明其作为包括骨骼肌在内的各种组织的抗衰老剂的有效性，皮肤,和脂肪组织。
