PDF(Pubmed)
Abstract：
Cancer therapy, from malignant tumor inhibition to cellular eradication treatment, remains a challenge, especially regarding reduced side effects and low energy consumption during treatment. Hence, phytochemicals as cytotoxic sensitizers or photosensitizers deserve special attention. The dark and photo-response of Yemenite \'Etrog\' leaf extracts applied to prostate PC3 cancer cells is reported here. An XTT cell viability assay along with light microscope observations revealed pronounced cytotoxic activity of the extract for long exposure times of 72 h upon concentrations of 175 μg/mL and 87.5 μg/mL, while phototoxic effect was obtained even at low concentration of 10.93 μg/mL and a short introduction period of 1.5 h. For the longest time incubation of 72 h and for the highest extract concentration of 175 μg/mL, relative cell survival decreased by up to 60% (below the IC50). In combined phyto-photodynamic therapy, a reduction of 63% compared to unirradiated controls was obtained. The concentration of extract in cells versus the accumulation time was inversely related to fluorescence emission intensity readings. Extracellular ROS production was also shown. Based on an ATR-FTIR analysis of the powdered leaves and their liquid ethanolic extract, biochemical fingerprints of both polar and non-polar phyto-constituents were identified, thereby suggesting their implementation as phyto-medicine and phyto-photomedicine.
摘要：
癌症治疗,从恶性肿瘤抑制到细胞根除治疗，仍然是一个挑战，特别是在治疗期间减少副作用和低能耗。因此，作为细胞毒性敏化剂或光敏剂的植物化学物质值得特别注意。此处报道了也门“Etrog”叶提取物应用于前列腺PC3癌细胞的黑暗和光响应。XTT细胞活力测定以及光学显微镜观察显示，在175μg/mL和87.5μg/mL的浓度下，提取物在72小时的长暴露时间内具有明显的细胞毒性活性。而即使在10.93μg/mL的低浓度和1.5h的短引入期下也能获得光毒性作用。对于72h的最长孵育时间和175μg/mL的最高提取物浓度，相对细胞存活率降低高达60%(低于IC50)。在联合植物光动力疗法中，与未照射的对照相比，减少了63%。细胞中提取物的浓度相对于积累时间与荧光发射强度读数成反比。还显示了细胞外ROS的产生。基于ATR-FTIR分析的粉末叶及其液体乙醇提取物，鉴定了极性和非极性植物成分的生化指纹图谱，从而表明它们作为植物药和植物药的实施。
