PDF(Pubmed)
Abstract：
The development of new drugs derived from plant sources is of significant interest in modern pharmacy. One of the promising plant sources for introduction into pharmaceuticals is Tripleurospermum inodorum (L.) Sch. Bip., also known as Tripleurospermum perforatum (Merat.) M. This plant has been shown to possess various biological activities, including anti-inflammatory, antimicrobial, and antimycotic activities, among others. However, a review of the current literature reveals a paucity of studies investigating the chemical composition of the herb Tripleurospermum inodorum (L.) Sch. Bip. This study presents the development of a method for obtaining an extract of the herb Tripleurospermum inodorum (L.) Sch. Bip. enriched with flavonoids, harvested before flowering and butonization. This study focused on determining the optimal conditions for extraction, including the concentration of the extractant (ethanol), extraction time, raw material/extractant ratio, extraction frequency, complexation reaction time, amount of aluminum chloride solution, and amount of diluted acetic acid. The results indicate that herbs harvested during this specific period exhibited a higher flavonoid content compared to those collected during butonization and flowering. Moreover, this study demonstrated that the flavonoid content could exceed 7% mg REq/100 g D.W. through a one-hour extraction process. Furthermore, the flavonoid content was found to be 7.65 ± 0.03 mg REq/100 g D.W. following a three-minute ultrasound-assisted extraction process, followed by thermal extraction. A qualitative analysis identified a variety of phenolic compounds in the extract, such as chlorogenic acid, 5-O-p-coumaroylquinic acid, 1-O-p-coumaroylquinic acid, luteolin-7-glucoside, quercetin-3-glucoside, luteolin-7-rutinoside, 3,5-O-dicaffeoylquinic acid, quercetin-3-O-malonylglucoside, apigenin-7-glucoside, luteolin-3-malonylglucoside, cynarin, rhamnetin-3-(O-dimethyl rhamnosyl glucosylglucoside), and luteolin. Moreover, this study demonstrated the antimicrobial, anti-inflammatory, anticoagulant, anti-aggregation, and antioxidant activities of the aqueous alcoholic extract from T. inodorum herb (ETIH) against pathogens such as Staphylococcus aureus, Escherichia coli, and Candida albicans. Additionally, the extract exhibited comparable anti-inflammatory effects on diclofenac sodium. These findings contribute to the understanding of the potential pharmacological applications of the developed herb extract.
摘要：
来自植物来源的新药的开发在现代药学中具有重要意义。引入药物的有希望的植物来源之一是Tripleurospermuminodorum（L.）Sch。Bip.,也被称为贯叶三叶草（Merat。)M.该植物已被证明具有各种生物活性，包括消炎药,抗菌，和抗真菌活性，在其他人中。然而,对现有文献的回顾表明，研究草本植物Tripleurospermuminodorum的化学成分的研究很少（L.）Sch。Bip.这项研究提出了一种获得草本植物Tripleurospermuminodorum提取物的方法的开发（L.）Sch。Bip.富含类黄酮，在开花和羽化之前收获。这项研究的重点是确定提取的最佳条件，包括萃取剂（乙醇）的浓度，提取时间,原料/萃取剂比例，提取频率,络合反应时间,氯化铝溶液的量，和稀释乙酸的量。结果表明，与羽化和开花期间收集的草药相比，在此特定时期收获的草药表现出更高的类黄酮含量。此外，这项研究表明，通过一小时的提取过程，类黄酮含量可以超过7％mgREq/100gD.W.。此外，在经过三分钟的超声辅助提取过程后，发现类黄酮含量为7.65±0.03mgREq/100gD.W.。其次是热提取。定性分析确定了提取物中的各种酚类化合物，如绿原酸，5-O-对-香豆酰基奎尼酸,1-O-对-香豆酰基奎尼酸,木犀草素-7-葡萄糖苷，槲皮素-3-葡萄糖苷，木犀草素-7-胡桃苷,3,5-O-二咖啡酰基奎尼酸,槲皮素-3-O-丙二酰葡萄糖苷，芹菜素-7-葡萄糖苷，木犀草素-3-丙二酰葡萄糖苷，cynarin,鼠李素-3-（O-二甲基鼠李糖基葡萄糖苷），和木犀草素.此外，这项研究证明了抗菌药物，抗炎,抗凝剂,反聚集，和抗氧化活性的含水醇提取物从T.inodorum草本植物（ETIH）对病原体，如金黄色葡萄球菌，大肠杆菌,和白色念珠菌.此外,该提取物对双氯芬酸钠具有相当的抗炎作用。这些发现有助于理解开发的草药提取物的潜在药理学应用。
