PDF(Pubmed)
Abstract：
BACKGROUND: The imbalance of skin microbiota in acne can induce changes leading to induction or to aggravation of chronic inflammatory lesions; complex mechanisms are involved. Cutibacterium acnes (C. acnes) ribotypes RT4 and RT5 express more biofilm and are associated with inflammatory acne lesions. C. acnes RT6 is a non-acne ribotype, beneficial for the skin.
OBJECTIVE: In an open clinical trial, acne adults were included and assessed clinically at baseline and at month 2 using the Investigator Global Assessment of Acne (IGA) score. A topical emulsion was applied twice daily for 2 months (M2) in each included patient. In the same series of acne patients, skin swab samples were collected from acne patients at baseline and M2 from lesional and non-lesional skin; skin swabs were collected for the metagenomic long-read analysis of microbiota.
METHODS: Acne patients with a gravity score IGA of >1<3 were included in this pilot study. An emulsion of O/W formulated with vegetal extract of Umbelliferae associated with a polysaccharide at 1% was applied twice daily for 2 months. At baseline and M2 clinical assessments were made; skin swab samples were also taken for microbiota analysis from lesional and non-lesional skin in each included patient. Extractions of genomic DNA (gDNA) from swab samples from baseline and from M2 were made, followed by full-length (V1-V9) amplification of the 16S rDNA and sequencing of amplicon libraries for strain-level bacterial community profiling.
RESULTS: In a series of 32 adult acne patients, the mean initial IGA scale was 3.1; at M2 the IGA scale was 1.5 (p < 0.001). The mean decrease in acne lesions was by 63%. Microbiome metagenomic long-read analysis in these series was mainly dominated by C. acnes followed by Staphylococcus epidermidis (S. epidermidis). The density of C. acnes ribotypes RT6 (non-acne strain) was increased at M2 compared to baseline and the density of ribotypes C. acnes RT1 to RT5 was decreased at M2, compared to baseline (p < 0.0001). S. epidermidis ribotypes (1 to 36) were non significantly increased at M2, compared to baseline (p < 0.1).
CONCLUSIONS: In a series of 32 acne patients that applied an emulsion based on vegetal extract of Umbelliferae and a polysaccharide at 1% twice daily, a significant clinical improvement in IGA scale for acne lesions was seen at M2, compared to baseline (p < 0.0001). The clinical improvement was correlated with an improvement in skin microbiome at M2 compared to baseline, indicated by the increase in the relative abundance of non-acne strain of C. acnes ribotype 6 and of the decrease in the relative abundance of acne strains ribotypes C. acnes RT1 to RT5.
OBJECTIVE: In an open clinical trial, acne adults were included and assessed clinically at baseline and at month 2 using the Investigator Global Assessment of Acne (IGA) score. A topical emulsion was applied twice daily for 2 months (M2) in each included patient. In the same series of acne patients, skin swab samples were collected from acne patients at baseline and M2 from lesional and non-lesional skin; skin swabs were collected for the metagenomic long-read analysis of microbiota.
METHODS: Acne patients with a gravity score IGA of >1<3 were included in this pilot study. An emulsion of O/W formulated with vegetal extract of Umbelliferae associated with a polysaccharide at 1% was applied twice daily for 2 months. At baseline and M2 clinical assessments were made; skin swab samples were also taken for microbiota analysis from lesional and non-lesional skin in each included patient. Extractions of genomic DNA (gDNA) from swab samples from baseline and from M2 were made, followed by full-length (V1-V9) amplification of the 16S rDNA and sequencing of amplicon libraries for strain-level bacterial community profiling.
RESULTS: In a series of 32 adult acne patients, the mean initial IGA scale was 3.1; at M2 the IGA scale was 1.5 (p < 0.001). The mean decrease in acne lesions was by 63%. Microbiome metagenomic long-read analysis in these series was mainly dominated by C. acnes followed by Staphylococcus epidermidis (S. epidermidis). The density of C. acnes ribotypes RT6 (non-acne strain) was increased at M2 compared to baseline and the density of ribotypes C. acnes RT1 to RT5 was decreased at M2, compared to baseline (p < 0.0001). S. epidermidis ribotypes (1 to 36) were non significantly increased at M2, compared to baseline (p < 0.1).
CONCLUSIONS: In a series of 32 acne patients that applied an emulsion based on vegetal extract of Umbelliferae and a polysaccharide at 1% twice daily, a significant clinical improvement in IGA scale for acne lesions was seen at M2, compared to baseline (p < 0.0001). The clinical improvement was correlated with an improvement in skin microbiome at M2 compared to baseline, indicated by the increase in the relative abundance of non-acne strain of C. acnes ribotype 6 and of the decrease in the relative abundance of acne strains ribotypes C. acnes RT1 to RT5.
摘要：
背景：痤疮中皮肤微生物群的失衡可引起改变，导致慢性炎性病变的诱导或加重；涉及复杂的机制。痤疮切杆菌(C.acnes)ribotypesRT4和RT5表达更多的生物膜，并与炎性痤疮病变相关。C.痤疮RT6是一种非痤疮核糖型，对皮肤有益。
目的：在一项开放的临床试验中，纳入成人痤疮患者,并在基线和第2个月时使用研究者全球痤疮评估(IGA)评分进行临床评估.在每个包括的患者中每天两次施用局部乳液,持续2个月(M2)。在同一系列痤疮患者中，基线时从痤疮患者收集皮肤拭子样本,从病灶和非病灶皮肤收集M2样本;收集皮肤拭子用于微生物区系的宏基因组长读分析.
方法：本试验研究包括重力评分IGA>1<3的痤疮患者。每天两次施用与1％的多糖缔合的伞形科植物提取物配制的O/W乳液，持续2个月。在基线和M2时进行临床评估;还从每个包括的患者的病变和非病变皮肤采集皮肤拭子样品用于微生物群分析。从基线和M2的拭子样品中提取基因组DNA（gDNA），然后对16SrDNA进行全长（V1-V9）扩增，并对扩增子文库进行测序，以进行菌株水平的细菌群落分析。
结果：在一系列32名成年痤疮患者中，平均初始IGA量表为3.1;在M2时,IGA量表为1.5(p<0.001)。痤疮病变的平均减少了63％。这些系列中的微生物组宏基因组长读分析主要由痤疮梭菌主导，其次是表皮葡萄球菌（S.表皮）。与基线相比,在M2处痤疮梭菌RbotypesRT6(非痤疮菌株)的密度增加,并且与基线相比,在M2处痤疮梭菌RT1至RT5的密度降低(p<0.0001)。与基线(p<0.1)相比,在M2时表皮葡萄球菌(1至36)无显著增加。
结论：在一系列32名痤疮患者中，每天两次使用基于伞形科植物提取物和1％多糖的乳液，与基线相比,M2时痤疮病变的IGA评分有显著临床改善(p<0.0001).与基线相比，临床改善与M2时皮肤微生物组的改善相关，由痤疮梭菌Rbottype6的非痤疮菌株的相对丰度的增加和痤疮菌株RbotypesC.acnesRT1至RT5的相对丰度的降低所表明。
目的：在一项开放的临床试验中，纳入成人痤疮患者,并在基线和第2个月时使用研究者全球痤疮评估(IGA)评分进行临床评估.在每个包括的患者中每天两次施用局部乳液,持续2个月(M2)。在同一系列痤疮患者中，基线时从痤疮患者收集皮肤拭子样本,从病灶和非病灶皮肤收集M2样本;收集皮肤拭子用于微生物区系的宏基因组长读分析.
方法：本试验研究包括重力评分IGA>1<3的痤疮患者。每天两次施用与1％的多糖缔合的伞形科植物提取物配制的O/W乳液，持续2个月。在基线和M2时进行临床评估;还从每个包括的患者的病变和非病变皮肤采集皮肤拭子样品用于微生物群分析。从基线和M2的拭子样品中提取基因组DNA（gDNA），然后对16SrDNA进行全长（V1-V9）扩增，并对扩增子文库进行测序，以进行菌株水平的细菌群落分析。
结果：在一系列32名成年痤疮患者中，平均初始IGA量表为3.1;在M2时,IGA量表为1.5(p<0.001)。痤疮病变的平均减少了63％。这些系列中的微生物组宏基因组长读分析主要由痤疮梭菌主导，其次是表皮葡萄球菌（S.表皮）。与基线相比,在M2处痤疮梭菌RbotypesRT6(非痤疮菌株)的密度增加,并且与基线相比,在M2处痤疮梭菌RT1至RT5的密度降低(p<0.0001)。与基线(p<0.1)相比,在M2时表皮葡萄球菌(1至36)无显著增加。
结论：在一系列32名痤疮患者中，每天两次使用基于伞形科植物提取物和1％多糖的乳液，与基线相比,M2时痤疮病变的IGA评分有显著临床改善(p<0.0001).与基线相比，临床改善与M2时皮肤微生物组的改善相关，由痤疮梭菌Rbottype6的非痤疮菌株的相对丰度的增加和痤疮菌株RbotypesC.acnesRT1至RT5的相对丰度的降低所表明。
