PDF(Pubmed)
Abstract：
The skin of bony fish is the first physical barrier and is responsible for maintaining the integrity of the fish. Lesions make the skin vulnerable to potential infection by pathogens present in the aquatic environment. In this way, wound repair has barely been studied in gilthead sea bream. Thus, this study investigated the modulation of peripheral neuro-endocrine and tissue repair markers at the transcriptional level in the skin of teleost fish subjected to mechanical damage above or below the lateral line (dorsal and ventral lesions, respectively). Samples were evaluated using RT-qPCR at 2-, 4-, and 20-days post-injury. Fish with a ventral lesion presented a trend of progressive increase in the expressions of corticotropin-releasing hormone (crh), pro-opiomelanocortin-A (pomca), proenkephalin-B (penkb), cholecystokinin (cck), oxytocin (oxt), angiotensinogen (agt), and (less pronounced) somatostatin-1B (sst1b). By contrast, fish with a dorsal lesion registered no significant increase or biological trend for the genes evaluated at the different sampling times. Collectively, the results show a rapid and more robust response of neuro-endocrine and tissue repair markers in the injuries below than above the lateral line, which could be attributable to their proximity to vital organs.
摘要：
硬骨鱼的皮肤是第一个物理屏障，负责保持鱼的完整性。病变使皮肤容易受到水生环境中存在的病原体的潜在感染。这样,伤口修复几乎没有在金头鱼中进行过研究。因此，这项研究调查了硬骨鱼皮肤在转录水平上的周围神经内分泌和组织修复标志物的调节受到侧线上方或下方的机械损伤（背侧和腹侧病变，分别)。使用RT-qPCR在2-，4-,受伤后20天。腹侧病变的鱼类促肾上腺皮质激素释放激素（crh）的表达呈逐渐增加的趋势，pro-opiomelanocortin-A（pomca），原脑啡肽-B（penkb），胆囊收缩素（cck），催产素（oxt），血管紧张素原（agt），和（不太明显）生长抑素-1B（sst1b）。相比之下,在不同采样时间评估的基因中，具有背侧病变的鱼没有显着增加或生物学趋势。总的来说，结果表明，神经内分泌和组织修复标志物在侧线以下的损伤中的反应比在侧线以上的快速和更强大，这可能归因于它们靠近重要器官。
