PDF(Pubmed)
Abstract：
Silicon included in a restructured meat (RM) matrix (Si-RM) as a functional ingredient has been demonstrated to be a potential bioactive antidiabetic compound. However, the jejunal and hepatic molecular mechanisms by which Si-RM exerts its cholesterol-lowering effects remain unclear. Male Wistar rats fed an RM included in a high-saturated-fat high-cholesterol diet (HSFHCD) combined with a low dose of streptozotocin plus nicotinamide injection were used as late-stage type 2 diabetes mellitus (T2DM) model. Si-RM was included into the HSFHCD as a functional food. An early-stage TD2M group fed a high-saturated-fat diet (HSFD) was taken as reference. Si-RM inhibited the hepatic and intestinal microsomal triglyceride transfer protein (MTP) reducing the apoB-containing lipoprotein assembly and cholesterol absorption. Upregulation of liver X receptor (LXRα/β) by Si-RM turned in a higher low-density lipoprotein receptor (LDLr) and ATP-binding cassette transporters (ABCG5/8, ABCA1) promoting jejunal cholesterol efflux and transintestinal cholesterol excretion (TICE), and facilitating partially reverse cholesterol transport (RCT). Si-RM decreased the jejunal absorptive area and improved mucosal barrier integrity. Consequently, plasma triglycerides and cholesterol levels decreased, as well as the formation of atherogenic lipoprotein particles. Si-RM mitigated the dyslipidemia associated with late-stage T2DM by Improving cholesterol homeostasis. Silicon could be used as an effective nutritional approach in diabetic dyslipidemia management.
摘要：
已证明包含在重组肉（RM）基质（Si-RM）中的硅作为功能成分是潜在的生物活性抗糖尿病化合物。然而,Si-RM发挥其降胆固醇作用的空肠和肝脏分子机制尚不清楚。饲喂包含在高饱和脂肪高胆固醇饮食（HSFHCD）中的RM的雄性Wistar大鼠与低剂量的链脲佐菌素加烟酰胺注射液一起用作晚期2型糖尿病（T2DM）模型。Si-RM作为功能性食品包含在HSFHCD中。以高饱和脂肪饮食（HSFD）的早期TD2M组作为参考。Si-RM抑制肝和肠微粒体甘油三酯转移蛋白（MTP），从而减少含apoB的脂蛋白组装和胆固醇吸收。通过Si-RM上调肝X受体（LXRα/β），转化为更高的低密度脂蛋白受体（LDLr）和ATP结合盒转运蛋白（ABCG5/8，ABCA1），促进空肠胆固醇流出和经肠胆固醇排泄（TICE），并促进部分反向胆固醇转运（RCT）。Si-RM减少空肠吸收面积并改善粘膜屏障完整性。因此,血浆甘油三酯和胆固醇水平下降，以及动脉粥样硬化脂蛋白颗粒的形成。Si-RM通过改善胆固醇稳态减轻与晚期T2DM相关的血脂异常。硅可以作为糖尿病血脂异常管理的有效营养方法。
