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Abstract：
The aim of this study was to test the molecular expression profile (senescence-associated secretory phenotype; SASP) in gingival crevicular fluid (GCF) prior to surgery in relation to the distribution of clinical success of periodontal regeneration. Forty consecutive patients presenting sites with residual probing pocket depth (PPD) ≥ 6 mm and intrabony defects ≥ 3 mm were treated through a minimally invasive surgical technique. Pre-operatively, GCF was sampled for inflammatory biomarker analysis related to SASP [interleukin (IL)-1β, IL-6, and IL-12; matrix-metalloproteinases (MMP)-8 and -9]. Better or worse responders were classified depending on the achievement of a composite outcome measure at 1-year [COM; PPD ≤ 4 mm and clinical attachment gain (CAL) gain ≥ 3 mm]. Correlation analyses and logistic regression models were performed. Periodontal regeneration led to significant improvements in mean clinical and radiographic parameters. Teeth achieving COM presented significantly lower amounts of SASP factors compared with non-successful teeth. Higher CAL gain, PPD reduction, and radiographic bone fill were negatively correlated with IL-1β and MMP-8 and -9 (p < 0.001), while IL-12 showed a direct relationship with CAL gain (p = 0.005) and PPD reduction (p = 0.038). Sites expressing higher SASP expression in the GCF before periodontal regeneration achieved worse clinical and radiographic outcomes.
摘要：
这项研究的目的是测试手术前龈沟液（GCF）中与牙周再生临床成功分布有关的分子表达谱（衰老相关分泌表型；SASP）。通过微创手术技术治疗了40例连续出现残余探查袋深度（PPD）≥6mm和骨内缺损≥3mm的患者。术前,对GCF进行采样以进行与SASP相关的炎症生物标志物分析[白细胞介素（IL）-1β，IL-6和IL-12;基质金属蛋白酶(MMP)-8和-9]。根据1年复合结局指标的实现情况[COM;PPD≤4mm和临床依恋增益（CAL）增益≥3mm]，对反应者进行了分类。进行相关分析和logistic回归模型。牙周再生导致平均临床和影像学参数的显着改善。与未成功的牙齿相比，实现COM的牙齿显示出SASP因子的数量显着降低。更高的CAL增益，PPD减少,骨填充与IL-1β、MMP-8和-9呈负相关(p<0.001),而IL-12显示与CAL增加（p=0.005）和PPD减少（p=0.038）直接相关。牙周再生前GCF中表达较高SASP表达的位点的临床和影像学结果较差。
