PDF(Pubmed)
Abstract：
Magnoliae Flos (MF) is a medicinal herb widely employed in traditional medicine for relieving sinusitis, allergic rhinitis, headaches, and toothaches. Here, we investigated the potential preventive effects of MF extract (MFE) against 4-vinylcyclohexene diepoxide (VCD)-induced ovotoxicity in ovarian cells and a mouse model of premature ovarian insufficiency (POI). The cytoprotective effects of MFE were assessed using CHO-K1 or COV434 cells. In vivo, B6C3F1 female mice were intraperitoneally injected with VCD for two weeks to induce POI, while MFE was orally administered for four weeks, beginning one week before VCD administration. VCD led to a significant decline in the viabilities of CHO-K1 and COV434 cells and triggered excessive reactive oxygen species (ROS) production and apoptosis specifically in CHO-K1 cells. However, pretreatment with MFE effectively prevented VCD-induced cell death and ROS generation, while also activating the Akt signaling pathway. In vivo, MFE increased relative ovary weights, follicle numbers, and serum estradiol and anti-Müllerian hormone levels versus controls under conditions of ovary failure. Collectively, our results demonstrate that MFE has a preventive effect on VCD-induced ovotoxicity through Akt activation. These results suggest that MFE may have the potential to prevent and manage conditions such as POI and diminished ovarian reserve.
摘要：
木兰（MF）是一种在传统医学中广泛用于缓解鼻窦炎的草药，过敏性鼻炎,头痛,还有牙痛.这里,我们研究了MF提取物（MFE）对4-乙烯基环己烯二环氧化物（VCD）诱导的卵巢细胞卵黄毒性的潜在预防作用，并建立了过早卵巢功能不全（POI）的小鼠模型。使用CHO-K1或COV434细胞评估MFE的细胞保护作用。在体内,B6C3F1雌性小鼠腹腔注射VCD两周诱导POI,而MFE口服给药四周，从VCD管理前一周开始。VCD导致CHO-K1和COV434细胞的活力显着下降，并特别在CHO-K1细胞中引发了过量的活性氧（ROS）产生和凋亡。然而,用MFE预处理可有效防止VCD诱导的细胞死亡和ROS生成，同时还激活Akt信号通路。在体内,MFE增加相对卵巢重量，卵泡数,在卵巢衰竭的情况下，血清雌二醇和抗苗勒管激素水平与对照组相比。总的来说，我们的结果表明,MFE通过Akt激活对VCD诱导的卵毒性具有预防作用.这些结果表明，MFE可能具有预防和管理POI和卵巢储备减少等疾病的潜力。
