PDF(Pubmed)
Abstract：
BACKGROUND: A subset of patients affected by cutaneous squamous cell carcinoma (cSCC) can exhibit locally invasive or metastatic tumors. Different staging classification systems are currently in use for cSCC. However, precise patient risk stratification has yet to be reached in clinical practice. The study aims to identify specific histological and molecular parameters characterizing metastatic cSCC.
METHODS: Patients affected by metastatic and non-metastatic cSCC (controls) were included in the present study and matched for clinical and histological characteristics. Skin samples from primary tumors were revised for several histological parameters and also underwent gene expression profiling with a commercially available panel testing 770 different genes.
RESULTS: In total, 48 subjects were enrolled in the study (24 cases, 24 controls); 67 genes were found to be differentially expressed between metastatic and non-metastatic cSCC. Most such genes were involved in immune regulation, skin integrity, angiogenesis, cell migration and proliferation.
CONCLUSIONS: The combination of histological and molecular profiles of cSCCs allows the identification of features specific to metastatic cSCC, with potential implications for more precise patient risk stratification.
METHODS: Patients affected by metastatic and non-metastatic cSCC (controls) were included in the present study and matched for clinical and histological characteristics. Skin samples from primary tumors were revised for several histological parameters and also underwent gene expression profiling with a commercially available panel testing 770 different genes.
RESULTS: In total, 48 subjects were enrolled in the study (24 cases, 24 controls); 67 genes were found to be differentially expressed between metastatic and non-metastatic cSCC. Most such genes were involved in immune regulation, skin integrity, angiogenesis, cell migration and proliferation.
CONCLUSIONS: The combination of histological and molecular profiles of cSCCs allows the identification of features specific to metastatic cSCC, with potential implications for more precise patient risk stratification.
摘要：
背景：受皮肤鳞状细胞癌（cSCC）影响的患者亚组可以表现出局部侵袭性或转移性肿瘤。不同的分期分类系统当前用于cSCC。然而,在临床实践中尚未实现精确的患者风险分层.该研究旨在确定表征转移性cSCC的特定组织学和分子参数。
方法：本研究包括转移性和非转移性cSCC患者（对照），并对其临床和组织学特征进行匹配。对来自原发性肿瘤的皮肤样品的几个组织学参数进行修正,并且还用市售小组测试770个不同基因进行基因表达谱分析。
结果：总计，48名受试者被纳入研究(24例，24个对照）；发现有67个基因在转移性和非转移性cSCC之间差异表达。大多数这样的基因参与免疫调节，皮肤完整性，血管生成,细胞迁移和增殖。
结论：cSCC的组织学和分子谱的组合允许鉴定特定于转移性cSCC的特征，对更精确的患者风险分层有潜在的影响。
方法：本研究包括转移性和非转移性cSCC患者（对照），并对其临床和组织学特征进行匹配。对来自原发性肿瘤的皮肤样品的几个组织学参数进行修正,并且还用市售小组测试770个不同基因进行基因表达谱分析。
结果：总计，48名受试者被纳入研究(24例，24个对照）；发现有67个基因在转移性和非转移性cSCC之间差异表达。大多数这样的基因参与免疫调节，皮肤完整性，血管生成,细胞迁移和增殖。
结论：cSCC的组织学和分子谱的组合允许鉴定特定于转移性cSCC的特征，对更精确的患者风险分层有潜在的影响。
