PDF(Pubmed)
Abstract：
Grapevine varieties from \"Douro Superior\" (NE Portugal) experience high temperatures, solar radiation, and water deficit during the summer. This summer\'s stressful growing conditions induce nucleic acids, lipids, and protein oxidation, which cause cellular, physiological, molecular, and biochemical changes. Cell cycle anomalies, mitosis delay, or cell death may occur at the cellular level, leading to reduced plant productivity. However, the foliar application of kaolin (KL) can mitigate the impact of abiotic stress by decreasing leaf temperature and enhancing antioxidant defence. Hence, this study hypothesised that KL-treated grapevine plants growing in NE Portugal would reveal, under summer stressful growing conditions, higher progression and stability of the leaf mitotic cell cycle than the untreated (control) plants. KL was applied after veraison for two years. Leaves, sampled 3 and 5 weeks later, were cytogenetically, molecularly, and biochemically analysed. Globally, integrating these multidisciplinary data confirmed the decreased leaf temperature and enhanced antioxidant defence of the KL-treated plants, accompanied by an improved regularity and completion of the leaf cell cycle relative to the control plants. Nevertheless, the KL efficacy was significantly influenced by the sampling date and/or variety. In sum, the achieved results confirmed the hypothesis initially proposed.
摘要：
来自“DouroSuperior”（葡萄牙东北部）的葡萄品种经历高温，太阳辐射,夏季缺水。今年夏天的紧张生长条件诱导核酸，脂质，和蛋白质氧化，导致细胞，生理,分子，和生化变化。细胞周期异常,有丝分裂延迟，或者细胞死亡可能发生在细胞水平，导致工厂生产力下降。然而,高岭土（KL）的叶面施用可以通过降低叶片温度和增强抗氧化防御能力来减轻非生物胁迫的影响。因此，这项研究假设，在葡萄牙东北部生长的KL处理的葡萄植物会揭示，在夏季紧张的生长条件下，叶片有丝分裂细胞周期的进展和稳定性高于未处理(对照)植物。KL在veraison后应用了两年。叶子,3周和5周后取样，是细胞遗传学的，分子，并进行了生化分析。全球范围内,整合这些多学科数据证实了KL处理植物的叶片温度降低和抗氧化防御增强，与对照植物相比，叶细胞周期的规律性和完成性得到改善。然而，KL疗效受采样日期和/或品种的显著影响.总之,取得的结果证实了最初提出的假设。
