PDF(Pubmed)
Abstract：
Fritillaria cirrhosa and its relatives have been utilized in traditional Chinese medicine for many years and are under priority protection in China. Despite their medicinal and protective value, research on their phylogeny, genetic diversity, and divergence remains limited. Here, we investigate the chloroplast genome variation architecture of 46 samples of F. cirrhosa and its relatives collected from various regions, encompassing the majority of wild populations across diverse geographical areas. The results indicate abundant variations in 46 accessions including 1659 single-nucleotide polymorphisms and 440 indels. Six variable markers (psbJ, ndhD, ycf1, ndhG, trnT-trnL, and rpl32-trnL) were identified. Phylogenetic and network analysis, population structure analysis, and principal component analysis showed that the 46 accessions formed five clades with significant divergence, which were related to their geographical distribution. The regions spanning from the southern Hengduan Mountains to the Qinghai-Tibet Plateau exhibited the highest levels of genetic diversity. F. cirrhosa and its relatives may have suffered a genetic bottleneck and have a relatively low genetic diversity level. Moreover, geographical barriers and discrete patches may have accelerated population divergence. The study offers novel perspectives on the phylogeny, genetic diversity, and population structure of F. cirrhosa and its relatives, information that can inform conservation and utilization strategies in the future.
摘要：
川贝母及其亲属已在中药中使用多年，在中国受到优先保护。尽管它们具有药用和保护价值，研究它们的系统发育，遗传多样性,分歧仍然有限。这里,我们调查了从不同地区收集的46份肝硬化及其亲属样本的叶绿体基因组变异结构，涵盖了不同地理区域的大多数野生种群。结果表明，46个种质中存在丰富的变异，包括1659个单核苷酸多态性和440个indel。六个可变标记(psbJ，ndhD,ycf1,ndhG,trnT-trnL,和rpl32-trnL)进行鉴定。系统发育和网络分析，人口结构分析，主成分分析表明，这46个种质形成了五个分支，差异显著，这与它们的地理分布有关。从横断山脉南部到青藏高原的地区表现出最高的遗传多样性水平。F.肝硬化及其亲属可能遭受了遗传瓶颈，遗传多样性水平相对较低。此外，地理障碍和离散斑块可能加速人口分化。该研究提供了有关系统发育的新颖观点，遗传多样性,以及肝硬化及其亲属的种群结构，可以为未来的保护和利用策略提供信息。
