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Abstract：
The current investigation endeavors to identify differentially expressed alternatively spliced (DAS) genes that exhibit concordant expression with splicing factors (SFs) under diverse multifactorial abiotic stress combinations in Arabidopsis seedlings. SFs serve as the post-transcriptional mechanism governing the spatiotemporal dynamics of gene expression. The different stresses encompass variations in salt concentration, heat, intensive light, and their combinations. Clusters demonstrating consistent expression profiles were surveyed to pinpoint DAS/SF gene pairs exhibiting concordant expression. Through rigorous selection criteria, which incorporate alignment with documented gene functionalities and expression patterns observed in this study, four members of the serine/arginine-rich (SR) gene family were delineated as SFs concordantly expressed with six DAS genes. These regulated SF genes encompass cactin, SR1-like, SR30, and SC35-like. The identified concordantly expressed DAS genes encode diverse proteins such as the 26.5 kDa heat shock protein, chaperone protein DnaJ, potassium channel GORK, calcium-binding EF hand family protein, DEAD-box RNA helicase, and 1-aminocyclopropane-1-carboxylate synthase 6. Among the concordantly expressed DAS/SF gene pairs, SR30/DEAD-box RNA helicase, and SC35-like/1-aminocyclopropane-1-carboxylate synthase 6 emerge as promising candidates, necessitating further examinations to ascertain whether these SFs orchestrate splicing of the respective DAS genes. This study contributes to a deeper comprehension of the varied responses of the splicing machinery to abiotic stresses. Leveraging these DAS/SF associations shows promise for elucidating avenues for augmenting breeding programs aimed at fortifying cultivated plants against heat and intensive light stresses.
摘要：
当前的研究致力于鉴定在拟南芥幼苗中在多种多因素非生物胁迫组合下表现出与剪接因子（SF）一致表达的差异表达的选择性剪接（DAS）基因。SF作为控制基因表达时空动态的转录后机制。不同的应力包括盐浓度的变化，热,密集的光，和他们的组合。调查了表现出一致表达谱的簇，以查明表现出一致表达的DAS/SF基因对。通过严格的选择标准，与本研究中观察到的已记录的基因功能和表达模式进行比对，丝氨酸/富含精氨酸（SR）基因家族的四个成员被描述为与六个DAS基因一致表达的SF。这些受调节的SF基因包括cactin，SR1-like,SR30和SC35类。鉴定的一致表达的DAS基因编码不同的蛋白质，如26.5kDa的热休克蛋白，蛋白伴侣DnaJ,钾通道GORK,钙结合EF手家族蛋白，DEAD-boxRNA解旋酶,和1-氨基环丙烷-1-羧酸合酶6.在一致表达的DAS/SF基因对中,SR30/DEAD-boxRNA解旋酶,和SC35样/1-氨基环丙烷-1-羧酸合酶6成为有希望的候选者，需要进一步检查以确定这些SF是否协调相应DAS基因的剪接。这项研究有助于更深入地理解剪接机制对非生物胁迫的各种响应。利用这些DAS/SF关联显示出有望阐明增强育种计划的途径，这些育种计划旨在增强栽培植物免受高温和强光胁迫的能力。
