PDF(Pubmed)
Abstract：
The effects of tritium at low doses and low dose rates have received increasing attention due to recent developments in fusion energy and the associated risks of tritium releases into the environment. Mitochondria have been identified as a potential candidate for studying the effects of low-dose/low-dose-rate radiation, with extensive experimental results obtained using X-ray irradiation. In this study, irradiation experiments were conducted on normal B-lymphoblastoid cells using HTO at varying doses. When compared to X-ray irradiation, no significant differences in cell viability induced by different doses were observed. However, the results of ATP levels showed a significant difference between the irradiated sample at a dose of 500 mGy by tritium beta-rays and the sham-irradiated sample, while the levels obtained with X-ray irradiation were almost identical to the sham-irradiated sample. In contrast, ATP levels for both tritium beta-rays and X-rays at a dose of 1.0 Gy showed minimal differences compared to the sham-irradiated sample. Furthermore, distinct effects at 500 mGy were also confirmed in both ROS levels and apoptosis results obtained through tritium beta-ray irradiation. This suggests that mitochondria might be a potential sensitive target for investigating the effects of tritium beta-ray irradiation.
摘要：
由于聚变能的最新发展以及氚释放到环境中的相关风险，低剂量和低剂量率的氚的影响越来越受到关注。线粒体已被确定为研究低剂量/低剂量率辐射影响的潜在候选者，使用X射线辐照获得了广泛的实验结果。在这项研究中,使用不同剂量的HTO对正常B淋巴母细胞进行辐射实验。与X射线照射相比，不同剂量诱导的细胞活力无显著差异。然而,ATP水平的结果表明，以500mGy的剂量被triβ射线辐照的样品与假辐照的样品之间存在显着差异，而X射线照射获得的水平几乎与假照射样品相同。相比之下,与假照射样品相比，剂量为1.0Gy的triβ射线和X射线的ATP水平差异最小。此外，在500mGy时，通过rsβ射线照射获得的ROS水平和凋亡结果也证实了明显的作用。这表明线粒体可能是研究triβ射线照射作用的潜在敏感靶标。
