PDF(Pubmed)
Abstract：
Lysine acetylation of proteins plays a critical regulatory function in plants. A few advances have been made in the study of plant acetylproteome. However, until now, there have been few data on Rhododendron chrysanthum Pall. (R. chrysanthum). We analyzed the molecular mechanisms of photosynthesis and stress resistance in R. chrysanthum under UV-B stress. We measured chlorophyll fluorescence parameters of R. chrysanthum under UV-B stress and performed a multi-omics analysis. Based on the determination of chlorophyll fluorescence parameters, R. chrysanthum Y(NO) (Quantum yield of non-photochemical quenching) increased under UV-B stress, indicating that the plant was damaged and photosynthesis decreased. In the analysis of acetylated proteomics data, acetylated proteins were found to be involved in a variety of biological processes. Notably, acetylated proteins were significantly enriched in the pathways of photosynthesis and carbon fixation, suggesting that lysine acetylation modifications have an important role in these activities. Our findings suggest that R. chrysanthum has decreased photosynthesis and impaired photosystems under UV-B stress, but NPQ shows that plants are resistant to UV-B. Acetylation proteomics revealed that up- or down-regulation of acetylation modification levels alters protein expression. Acetylation modification of key enzymes of the Calvin cycle (Rubisco, GAPDH) regulates protein expression, making Rubisco and GAPDH proteins expressed as significantly different proteins, which in turn affects the carbon fixation capacity of R. chrysanthum. Thus, Rubisco and GAPDH are significantly differentially expressed after acetylation modification, which affects the carbon fixation capacity and thus makes the plant resistant to UV-B stress. Lysine acetylation modification affects biological processes by regulating the expression of key enzymes in photosynthesis and carbon fixation, making plants resistant to UV-B stress.
摘要：
蛋白质的赖氨酸乙酰化在植物中起着关键的调节功能。植物乙酰蛋白质组的研究取得了一些进展。然而,直到现在,关于雪花杜鹃花的数据很少。(R.chrysanthum）。我们分析了UV-B胁迫下小白草光合作用和抗逆性的分子机制。我们在UV-B胁迫下测量了小黄的叶绿素荧光参数，并进行了多组学分析。在测定叶绿素荧光参数的基础上,R.chrysanthumY（NO）（非光化学猝灭的量子产率）在UV-B胁迫下增加，表明植物受损，光合作用下降。在对乙酰化蛋白质组学数据的分析中，发现乙酰化蛋白参与多种生物过程。值得注意的是,乙酰化蛋白质在光合作用和碳固定途径中显著富集，表明赖氨酸乙酰化修饰在这些活动中具有重要作用。我们的发现表明R.chrysanthum在UV-B胁迫下减少了光合作用并损害了光系统，但是NPQ表明植物对UV-B具有抗性。乙酰化蛋白质组学显示，乙酰化修饰水平的上调或下调会改变蛋白质表达。卡尔文循环关键酶的乙酰化修饰(Rubisco，GAPDH）调节蛋白质表达，使Rubisco和GAPDH蛋白表达为显著不同的蛋白，这反过来又影响了R.chrysanthum的碳固定能力。因此，Rubisco和GAPDH在乙酰化修饰后显著差异表达,这影响了碳固定能力，从而使植物对UV-B胁迫具有抗性。赖氨酸乙酰化修饰通过调节光合作用和碳固定中关键酶的表达影响生物过程，使植物抵抗UV-B胁迫。
