PDF(Pubmed)
Abstract：
Multiple sclerosis (MS) is a chronic and progressive neurological disorder, characterized by neuroinflammation and demyelination within the central nervous system (CNS). The etiology and the pathogenesis of MS are still unknown. Till now, no satisfactory treatments, diagnostic and prognostic biomarkers are available for MS. Therefore, we aimed to investigate metabolic alterations in patients with MS compared to controls and across MS subtypes. Metabolic profiles of serum samples from patients with MS (n = 90) and healthy control (n = 30) were determined by Nuclear Magnetic Resonance (1H-NMR) Spectroscopy using cryogenic probe. This approach was also utilized to identify significant differences between the metabolite profiles of the MS groups (primary progressive, secondary progressive, and relapsing-remitting) and the healthy controls. Concentrations of nine serum metabolites (adenosine triphosphate (ATP), tryptophan, formate, succinate, glutathione, inosine, histidine, pantothenate, and nicotinamide adenine dinucleotide (NAD)) were significantly higher in patients with MS compared to control. SPMS serum exhibited increased pantothenate and tryptophan than in PPMS. In addition, lysine, myo-inositol, and glutamate exhibited the highest discriminatory power (0.93, 95% CI 0.869-0.981; 0.92, 95% CI 0.859-0.969; 0.91, 95% CI 0.843-0.968 respectively) between healthy control and MS. Using NMR- based metabolomics, we identified a set of metabolites capable of classifying MS patients and controls. These findings confirmed untargeted metabolomics as a useful approach for the discovery of possible novel biomarkers that could aid in the diagnosis of the disease.
摘要：
多发性硬化症（MS）是一种慢性和进行性神经系统疾病，以中枢神经系统（CNS）内的神经炎症和脱髓鞘为特征。MS的病因及发病机制尚不清楚。到现在为止,没有令人满意的治疗方法，诊断和预后生物标志物可用于MS。因此,我们旨在调查MS患者与对照组和MS亚型之间的代谢改变.使用低温探针通过核磁共振（1H-NMR）光谱法测定MS患者（n=90）和健康对照（n=30）的血清样品的代谢谱。此方法还用于确定MS组的代谢物谱之间的显着差异（原发性进行性，二级进步,和复发缓解）和健康对照。九种血清代谢物的浓度（三磷酸腺苷（ATP），色氨酸,甲酸盐,琥珀酸盐,谷胱甘肽,肌苷,组氨酸,泛酸,与对照组相比，MS患者的烟酰胺腺嘌呤二核苷酸（NAD）明显更高。SPMS血清显示泛酸和色氨酸比PPMS增加。此外,赖氨酸，肌醇,和谷氨酸在健康对照组和MS之间表现出最高的辨别力（分别为0.93，95％CI0.869-0.981;0.92，95％CI0.859-0.969;0.91，95％CI0.843-0.968）。使用基于NMR的代谢组学，我们确定了一组能够对MS患者和对照进行分类的代谢物.这些发现证实了非靶向代谢组学是发现可能有助于疾病诊断的新型生物标志物的有用方法。
