Abstract：
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production in various types of mammalian cell lines. We decided to establish BacMam in our lab in order to streamline our workflows for gene expression in insect and mammalian cells, as it is straightforward to parallelize the baculovirus generation for both types of eukaryotic cells. This chapter provides a step-by-step description of the protocols we use for the generation of the recombinant BacMam viruses, the transduction of mammalian cell cultures, and optimization of the protein production conditions through small-scale expression and purification tests.
摘要：
杆状病毒介导的哺乳动物细胞基因表达,BacMam,是用于在各种类型的哺乳动物细胞系中产生重组蛋白的瞬时转染的有用替代方法。我们决定在实验室中建立BacMam，以简化我们在昆虫和哺乳动物细胞中基因表达的工作流程，因为对两种类型的真核细胞并行化杆状病毒的产生是直接的。本章提供了我们用于产生重组BacMam病毒的方案的逐步描述，哺乳动物细胞培养的转导，并通过小规模表达和纯化试验优化蛋白生产条件。
