Abstract：
This study investigates nanostructured titanium surfaces (Ti2 spikes) that promote the viability of osteoblasts and fibroblasts and prevent bacterial colonisation. Helium ion irradiation was adopted to produce nanometric-sized cones on titanium. Human osteoblasts (hFOB) and human gingiva fibroblasts (hGF) were used for analysis. A viability and a cytotoxicity assay were conducted to evaluate the lactate dehydrogenase (LDH) activity and assess cell damage in Ti2 spikes compared to titanium discs with a sandblasted and acid-etched (Ti2 SLA) surface. The antibacterial activity was investigated against Escherichia coli, Streptococcus mutans, Fusobacterium nucleatum, and Porphyromonas gingivalis. In the course of the cultivation, both hGF and hFOB demonstrated significantly reduced viability on the Ti2 spikes surface. hGF cells exhibited a slight but significant increase in LDH release. In contrast, hFOB showed reduced cytotoxicity on this surface. On the Ti2 spikes surface, hGF cells exhibited a significant reduction in gene expression of VCL, Src-1, and ITGα5. However, the integrin subunits ITGα1 and ITGα3 showed upregulation on the Ti2 spikes surface. The Ti2 spikes surface significantly increased the expression of almost all osteogenic markers. The results of conventional culturing demonstrated a statistically significant decrease in the number of viable cells for S. mutans, F. nucleaum, and greater quantities of P. gingivalis on Ti2 spikes surface compared to control. However, no such reduction was detected for E. coli. The long-term success of implants relies on establishing and maintaining hard and soft peri-implant tissues. Ti2 spikes represent a novel and promising approach to enhance osseointegration and optimize biocompatibility.
摘要：
这项研究调查了纳米结构的钛表面（Ti2尖峰），可促进成骨细胞和成纤维细胞的活力并防止细菌定植。采用氦离子辐照在钛上产生纳米尺寸的锥体。人成骨细胞(hFOB)和人牙龈成纤维细胞(hGF)用于分析。与具有喷砂和酸蚀刻（Ti2SLA）表面的钛盘相比，进行了活力和细胞毒性测定以评估乳酸脱氢酶（LDH）活性并评估Ti2尖峰中的细胞损伤。对大肠杆菌的抗菌活性进行了研究，变形链球菌,具核梭杆菌,和牙龈卟啉单胞菌.在种植过程中,hGF和hFOB均显示在Ti2穗表面上的活力显著降低。hGF细胞在LDH释放方面表现出轻微但显著的增加。相比之下,hFOB在该表面上显示降低的细胞毒性。在Ti2尖峰表面上，hGF细胞显示VCL基因表达显著降低,Src-1和ITGα5。然而,整联蛋白亚基ITGα1和ITGα3在Ti2尖峰表面上显示出上调。Ti2尖峰表面显着增加了几乎所有成骨标志物的表达。常规培养的结果表明，变异链球菌的活细胞数量在统计学上显着减少，F.核子,并且与对照相比,在Ti2穗表面上更大量的牙龈卟啉单胞菌。然而,对于大肠杆菌没有检测到这种减少。植入物的长期成功依赖于建立和维持硬的和软的植入物周围组织。Ti2尖峰代表了增强骨整合和优化生物相容性的新颖且有前途的方法。
