PDF(Pubmed)
Abstract：
Microorganisms, including potential pathogens, can colonise plastic surfaces in aquatic environments. This study investigates the colonisation of plastic pellets by Escherichia coli (E. coli) as a proxy for faecal pathogens in aquatic environments. Plastic pellets from a polluted beach were placed in seawater aquaria spiked with E. coli. Diverse bacteria, primarily from the Proteobacteria phylum, rapidly colonised the pellets within 24 h, with notable species known for plastic or hydrocarbon degradation. Over 26 days, biofilms formed on the plastic surfaces, reaching bacterial populations of up to 6.8·105 gene copies (gc) of the 16S rRNA mm-2. E. coli, was detected in the pellets for up to 7 days using culture methods, exhibiting varying attachment densities regardless of source or environmental factors. The study highlights plastic biofilms as reservoirs for E. coli, contributing to the survival and persistence of faecal bacteria in aquatic systems. These findings deepen our understanding of the risks associated with plastic pollution in marine settings, offering insights into the behaviour of faecal indicators and their implications for water quality assessments, while providing valuable information on potential pathogen dissemination within plastic-associated microbial communities.
摘要：
微生物,包括潜在的病原体,可以在水生环境中定居塑料表面。这项研究调查了大肠杆菌（E.大肠杆菌)作为水生环境中粪便病原体的替代。来自污染海滩的塑料颗粒被放置在掺有大肠杆菌的海水水族馆中。不同的细菌,主要来自变形杆菌门，在24小时内迅速定植颗粒，具有以塑料或碳氢化合物降解而闻名的物种。超过26天,在塑料表面上形成的生物膜，达到16SrRNAmm-2的多达6.8·105基因拷贝（gc）的细菌种群。大肠杆菌，使用培养方法在颗粒中检测到长达7天，无论来源或环境因素如何，都表现出不同的附着密度。这项研究强调了塑料生物膜作为大肠杆菌的储库，有助于粪便细菌在水生系统中的存活和持久性。这些发现加深了我们对海洋环境中与塑料污染相关风险的理解。提供有关粪便指标行为及其对水质评估的影响的见解，同时提供有关塑料相关微生物群落内潜在病原体传播的有价值信息。
