METHODS: We included a total of 62 female patients, all of whom had undergone laparoscopic surgery. Of these, 47 had endometriosis and 15 did not. During surgery, we collected peritoneal fluid (PF) and peripheral blood (PB) samples from every patient for analysis using flow cytometry. To mark the samples, we used a panel of monoclonal antibodies and examined TIM-3 expression in their immune cells.
RESULTS: Endometriosis patients in PB demonstrated a significantly lower percentage of CD56+ T cells with TIM-3 expression. As endometriosis progressed through its stages, this expression lessened. This decrease was particularly notable in women with stage III/IV endometriosis. Additionally, both women diagnosed with intestinal endometriosis and those with recent endometriosis diagnoses showed a significantly reduced percentage of CD56+ T cells expressing TIM-3.
CONCLUSIONS: Patients with endometriosis exhibit diminished TIM-3 expression within circulating T cells. This warrants further investigation to discern whether it contributes to the progression of endometriosis, potentially through the amplification of autoreactive T cells and inflammation.
方法:我们共纳入62名女性患者,所有这些人都接受了腹腔镜手术。其中,47人患有子宫内膜异位症,15人没有。手术期间,我们收集了每位患者的腹膜液(PF)和外周血(PB)样本进行流式细胞术分析.要标记样品,我们使用了一组单克隆抗体,并检测了其免疫细胞中TIM-3的表达.
结果:PB中的子宫内膜异位症患者显示出具有TIM-3表达的CD56+T细胞百分比明显降低。随着子宫内膜异位症的发展,这个表达减少了。这种减少在患有III/IV期子宫内膜异位症的女性中尤为显著。此外,诊断为肠道子宫内膜异位症的女性和最近诊断为子宫内膜异位症的女性均显示表达TIM-3的CD56+T细胞百分比显著降低.
结论:子宫内膜异位症患者循环T细胞内TIM-3表达减少。这需要进一步的研究来辨别它是否有助于子宫内膜异位症的进展。可能通过自身反应性T细胞和炎症的扩增。