Abstract：
Retinitis pigmentosa is the main cause of inherited human blindness and is associated with dysfunctional photoreceptors (PRs). Compared with traditional methods, optoelectronic stimulation can better preserve the structural integrity and genetic content of the retina. However, enhancing the spatiotemporal accuracy of stimulation is challenging. Quantum dot-doped ZnIn2S4 microflowers (MF) are utilized to construct a biomimetic photoelectric interface with a 0D/3D heterostructure, aiming to restore the light response in PR-degenerative mice. The MF bio interface has dimensions similar to those of natural PRs and can be distributed within the curved spatial region of the retina, mimicking cellular dispersion. The soft 2D nano petals of the MF provide a large specific surface area for photoelectric activation and simulate the flexibility interfacing between cells. This bio interface can selectively restore the light responses of seven types of retina ganglion cells that encode brightness. The distribution of responsive cells forms a pattern similar to that of normal mice, which may reflect the generation of the initial \"neural code\" in the degenerative retina. Patch-clamp recordings indicate that the bio interface can induce spiking and postsynaptic currents at the single-neuron level. The results will shed light on the development of a potential bionic subretinal prosthetic toolkit for visual function restoration.
摘要：
色素性视网膜炎是遗传性人类失明的主要原因，并与功能失调的光感受器（PR）有关。与传统方法相比,光电刺激可以更好地保持视网膜的结构完整性和遗传含量。然而,增强刺激的时空准确性具有挑战性。量子点掺杂的ZnIn2S4微花（MF）用于构建具有0D/3D异质结构的仿生光电界面，旨在恢复PR退化小鼠的光反应。MF生物界面具有与天然PR相似的尺寸，并且可以分布在视网膜的弯曲空间区域内，模仿细胞分散。MF的软2D纳米花瓣为光电激活提供了大的比表面积，并模拟了细胞之间的柔性接口。这种生物界面可以选择性地恢复编码亮度的七种类型的视网膜神经节细胞的光响应。反应细胞的分布形成与正常小鼠相似的模式，这可能反映了退化视网膜中初始“神经密码”的产生。膜片钳记录表明，生物界面可以在单神经元水平上诱导尖峰和突触后电流。结果将阐明用于视觉功能恢复的潜在仿生视网膜下假体工具包的开发。
