PDF(Pubmed)
Abstract：
Arbuscular mycorrhizal (AM) fungi can enhance the uptake of soil nutrients and water by citrus, promoting its growth. However, the specific mechanisms underlying the action of AM fungi in promoting the growth of citrus were not fully elucidated. This study aimed to explore the role of AM fungi Funneliformis mosseae in the regulatory mechanisms of P. trifoliata growth. Pot experiments combined with non-targeted metabolomics methods were used to observe the growth process and changes in metabolic products of P. trifoliata under the conditions of F. mosseae inoculation. The results showed that F. mosseae could form an excellent symbiotic relationship with P. trifoliata, thereby enhancing the utilization of soil nutrients and significantly promoting its growth. Compared with the control, the plant height, stem diameter, number of leaves, and aboveground and underground dry weight in the F. mosseae inoculation significantly increased by 2.57, 1.29, 1.57, 4.25, and 2.78 times, respectively. Moreover, the root system results confirmed that F. mosseae could substantially promote the growth of P. trifoliata. Meanwhile, the metabolomics data indicated that 361 differential metabolites and 56 metabolic pathways were identified in the roots of P. trifoliata and were inoculated with F. mosseae. This study revealed that the inoculated F. mosseae could participate in ABC transporters by upregulating their participation, glycerophospholipid metabolism, aminoacyl tRNA biosynthesis, tryptophan metabolism and metabolites from five metabolic pathways of benzoxazinoid biosynthesis [mainly enriched in lipid (39.50%) and amino acid-related metabolic pathways] to promote the growth of P. trifoliata.
摘要：
丛枝菌根（AM）真菌可以增强柑橘对土壤养分和水分的吸收，促进其增长。然而,AM真菌促进柑橘生长的具体机制尚未完全阐明。本研究旨在探讨菌型AM真菌在三叶草生长调控机制中的作用。采用盆栽实验结合非靶向代谢组学方法,观察了接种莫来贝条件下三叶草的生长过程和代谢产物的变化。结果表明，苔藓能与三叶草形成良好的共生关系，从而提高土壤养分的利用率，显著促进其生长。与对照相比，植物高度,阀杆直径,叶子的数量,接种苔藓的地上和地下干重分别增加了2.57、1.29、1.57、4.25和2.78倍，分别。此外，根系结果证实，苔藓能显著促进三叶草的生长。同时,代谢组学数据表明，在三叶假单胞菌的根中鉴定出361种差异代谢物和56种代谢途径，并接种了假单胞菌。这项研究表明，接种的F.mosseae可以通过上调其参与来参与ABC转运蛋白，甘油磷脂代谢，氨酰tRNA生物合成，色氨酸代谢和代谢产物来自苯并恶嗪类生物合成的5个代谢途径[主要富集于脂质(39.50%)和氨基酸相关代谢途径],以促进三叶黄芩的生长。
