PDF(Pubmed)
Abstract：
There is a pressing need to enhance early detection methods of E. coli O157:H7 to mitigate the occurrence and consequences of pathogenic contamination and associated outbreaks. This study highlights the efficacy of a portable electrochemical sensing platform that operates without faradaic processes towards detecting and quantifying E. coli O157:H7. It is specifically tailored for quick identification in potable water. The assay processing time is approximately 5 min, addressing the need for swift and efficient pathogen detection. The sensing platform was constructed utilizing specific, monoclonal E. coli antibodies, based on single-capture, non-faradaic, electrochemical immunoassay principles. The E. coli sensor assay underwent testing over a wide concentration range, spanning from 10 to 105 CFU/mL, and a limit of detection (LoD) of 1 CFU/mL was demonstrated. Significantly, the sensor\'s performance remained consistent across studies, with both inter- and intra-study coefficients of variation consistently below 20%. To evaluate real-world feasibility, a comparative examination was performed between laboratory-based benchtop data and data obtained from the portable device. The proposed sensing platform exhibited remarkable sensitivity and selectivity, enabling the detection of minimal E. coli concentrations in potable water. This successful advancement positions it as a promising solution for prompt on-site detection, characterized by its portability and user-friendly operation. This study presents electrochemical-based sensors as significant contributors to ensuring food safety and public health. They play a crucial role in preventing the occurrence of epidemics and enhancing the supervision of water quality.
摘要：
迫切需要增强大肠杆菌O157:H7的早期检测方法,以减轻致病性污染和相关爆发的发生和后果。这项研究强调了便携式电化学传感平台的功效，该平台无需法拉第过程即可检测和定量大肠杆菌O157：H7。它是专门为快速识别饮用水。分析处理时间约为5分钟，满足快速高效病原体检测的需求。传感平台是利用特定的，单克隆大肠杆菌抗体，基于单次捕获，非法拉第,电化学免疫分析原理.大肠杆菌传感器分析在很宽的浓度范围内进行了测试，范围从10到105CFU/mL，并证明了检测限（LoD）为1CFU/mL。重要的是,传感器的性能在各个研究中保持一致，研究间和研究内的变异系数始终低于20%。为了评估现实世界的可行性，我们对基于实验室的台式数据和从便携式设备获得的数据进行了比较检查.所提出的传感平台表现出显著的灵敏度和选择性，能够检测饮用水中最小的大肠杆菌浓度。这种成功的进步使其成为迅速进行现场检测的有希望的解决方案，其特点是其便携性和用户友好的操作。这项研究提出了基于电化学的传感器作为确保食品安全和公众健康的重要贡献者。它们在预防流行病的发生和加强水质监督方面发挥着至关重要的作用。
