PDF(Pubmed)
Abstract：
A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16 × 16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement.
摘要：
描述了一种用于分子诊断的即时护理系统（PoC-MD），结合GaN和CMOS芯片。该装置是荧光测量的微系统，能够分析强度和寿命。它由基于32×32矩阵可寻址GaNmicroLED阵列的混合微结构组成，具有50µm边长和100µm间距的方形LED，下面的线接合定制芯片集成了它们的驱动器，并与16×16单光子雪崩二极管（SPAD）CMOS阵列面对面放置。这种方法取代了基于激光的仪器，笨重的光学元件，和具有全混合微系统的分立电子器件，实现对32×32点的测量。报告的系统适用于长寿命（>10ns）荧光团，检出限为〜1/4µM。证明了链霉亲和素缀合物Qdot™605和氨基PEGQdot™705的概念验证测量，随着设备在同一测量中检测两个荧光团的能力。
