{Reference Type}: Journal Article
{Title}: Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array.
{Author}: Moro V;Canals J;Moreno S;Higgins-Wood S;Alonso O;Waag A;Prades JD;Dieguez A;
{Journal}: Biosensors (Basel)
{Volume}: 14
{Issue}: 6
{Year}: 2024 May 22
{Factor}: 5.743
{DOI}: 10.3390/bios14060264
{Abstract}: A Point-of-Care system for molecular diagnosis (PoC-MD) is described, combining GaN and CMOS chips. The device is a micro-system for fluorescence measurements, capable of analyzing both intensity and lifetime. It consists of a hybrid micro-structure based on a 32 × 32 matrix addressable GaN microLED array, with square LEDs of 50 µm edge length and 100 µm pitch, with an underneath wire bonded custom chip integrating their drivers and placed face-to-face to an array of 16 × 16 single-photon avalanche diodes (SPADs) CMOS. This approach replaces instrumentation based on lasers, bulky optical components, and discrete electronics with a full hybrid micro-system, enabling measurements on 32 × 32 spots. The reported system is suitable for long lifetime (>10 ns) fluorophores with a limit of detection ~1/4 µM. Proof-of-concept measurements of streptavidin conjugate Qdot™ 605 and Amino PEG Qdot™ 705 are demonstrated, along with the device ability to detect both fluorophores in the same measurement.