Abstract：
Osseointegration is the most important factor determining implant success. The surface modification of TiO2 nanotubes prepared by anodic oxidation has remarkable advantages in promoting bone formation. However, the mechanism behind this phenomenon is still unintelligible. Here we show that the nanomorphology exhibited open and clean nanotube structure and strong hydrophilicity, and the nanomorphology significantly facilitated the adhesion, proliferation, and osteogenesis differentiation of stem cells. Exploring the mechanism, we found that the nanomorphology can enhance mitochondrial oxidative phosphorylation (OxPhos) by activating Piezo1 and increasing intracellular Ca2+. The increase in OxPhos can significantly uplift the level of acetyl-CoA in the cytoplasm but not significantly raise the level of acetyl-CoA in the nucleus, which was beneficial for the acetylation and stability of β-catenin and ultimately promoted osteogenesis. This study provides a new interpretation for the regulatory mechanism of stem cell osteogenesis by nanomorphology.
摘要：
骨整合是决定植入成功的最重要因素。通过阳极氧化制备的TiO2纳米管的表面改性在促进骨形成方面具有显著的优势。然而,这种现象背后的机制仍然难以理解。在这里，我们表明纳米形貌表现出开放和干净的纳米管结构和强亲水性，纳米形态显著促进了粘附，扩散,和干细胞的成骨分化。探索机制，我们发现纳米形态可以通过激活Piezo1和增加细胞内Ca2+来增强线粒体氧化磷酸化(OxPhos)。OxPhos的增加可以显着提高细胞质中乙酰辅酶A的水平，但不会显着提高细胞核中乙酰辅酶A的水平。这有利于β-catenin的乙酰化和稳定性，并最终促进成骨。本研究为纳米形态学研究干细胞成骨的调控机制提供了新的解释。
