PDF(Pubmed)
Abstract：
UNASSIGNED: The aim of the study was to evaluate the modulating effects of five commonly used sweetener (glucose, inulin, isomaltulose, tagatose, trehalose) containing mouth rinses on the oral microbiome.
UNASSIGNED: A single-centre, double-blind, parallel randomized clinical trial was performed with healthy, 18-55-year-old volunteers (N = 65), who rinsed thrice-daily for two weeks with a 10% solution of one of the allocated sweeteners. Microbiota composition of supragingival dental plaque and the tongue dorsum coating was analysed by 16S RNA gene amplicon sequencing of the V4 hypervariable region (Illumina MiSeq). As secondary outcomes, dental plaque red fluorescence and salivary pH were measured.
UNASSIGNED: Dental plaque microbiota changed significantly for two groups: inulin (F = 2.0239, p = 0.0006 PERMANOVA, Aitchison distance) and isomaltulose (F = 0.67, p = 0.0305). For the tongue microbiota, significant changes were observed for isomaltulose (F = 0.8382, p = 0.0452) and trehalose (F = 1.0119, p = 0.0098). In plaque, 13 species changed significantly for the inulin group, while for tongue coating, three species changed for the trehalose group (ALDEx2, p < 0.1). No significant changes were observed for the secondary outcomes.
UNASSIGNED: The effects on the oral microbiota were sweetener dependant with the most pronounced effect on plaque microbiota. Inulin exhibited the strongest microbial modulating potential of the sweeteners tested. Further full-scale clinical studies are required.
UNASSIGNED: A single-centre, double-blind, parallel randomized clinical trial was performed with healthy, 18-55-year-old volunteers (N = 65), who rinsed thrice-daily for two weeks with a 10% solution of one of the allocated sweeteners. Microbiota composition of supragingival dental plaque and the tongue dorsum coating was analysed by 16S RNA gene amplicon sequencing of the V4 hypervariable region (Illumina MiSeq). As secondary outcomes, dental plaque red fluorescence and salivary pH were measured.
UNASSIGNED: Dental plaque microbiota changed significantly for two groups: inulin (F = 2.0239, p = 0.0006 PERMANOVA, Aitchison distance) and isomaltulose (F = 0.67, p = 0.0305). For the tongue microbiota, significant changes were observed for isomaltulose (F = 0.8382, p = 0.0452) and trehalose (F = 1.0119, p = 0.0098). In plaque, 13 species changed significantly for the inulin group, while for tongue coating, three species changed for the trehalose group (ALDEx2, p < 0.1). No significant changes were observed for the secondary outcomes.
UNASSIGNED: The effects on the oral microbiota were sweetener dependant with the most pronounced effect on plaque microbiota. Inulin exhibited the strongest microbial modulating potential of the sweeteners tested. Further full-scale clinical studies are required.
摘要：
■该研究的目的是评估五种常用甜味剂（葡萄糖，菊粉,异麦芽酮糖,塔格糖,海藻糖）含有口腔微生物组上的漱口水。
■单中心，双盲,平行随机临床试验与健康，18-55岁的志愿者（N=65），他每天用10%的一种甜味剂溶液冲洗三次，持续两周。通过V4高变区（IlluminaMiSeq）的16SRNA基因扩增子测序分析了牙龈上牙菌斑和舌背涂层的微生物组成。作为次要结果，测量牙菌斑红色荧光和唾液pH。
■两组的牙菌斑菌群发生了显着变化：菊粉（F=2.0239，p=0.0006PERMANOVA，Aitchison距离）和异麦芽酮糖（F=0.67，p=0.0305）。对于舌头微生物群，异麦芽酮糖(F=0.8382,p=0.0452)和海藻糖(F=1.0119,p=0.0098)均有显著变化。在牌匾上，菊粉组有13种显著变化，而舌苔，海藻糖组改变了三个物种（ALDEx2，p<0.1）。次要结果没有观察到显著变化。
■对口腔微生物群的影响是甜味剂依赖性的,对斑块微生物群的影响最显著。菊粉在所测试的甜味剂中表现出最强的微生物调节潜力。需要进一步的全面临床研究。
■单中心，双盲,平行随机临床试验与健康，18-55岁的志愿者（N=65），他每天用10%的一种甜味剂溶液冲洗三次，持续两周。通过V4高变区（IlluminaMiSeq）的16SRNA基因扩增子测序分析了牙龈上牙菌斑和舌背涂层的微生物组成。作为次要结果，测量牙菌斑红色荧光和唾液pH。
■两组的牙菌斑菌群发生了显着变化：菊粉（F=2.0239，p=0.0006PERMANOVA，Aitchison距离）和异麦芽酮糖（F=0.67，p=0.0305）。对于舌头微生物群，异麦芽酮糖(F=0.8382,p=0.0452)和海藻糖(F=1.0119,p=0.0098)均有显著变化。在牌匾上，菊粉组有13种显著变化，而舌苔，海藻糖组改变了三个物种（ALDEx2，p<0.1）。次要结果没有观察到显著变化。
■对口腔微生物群的影响是甜味剂依赖性的,对斑块微生物群的影响最显著。菊粉在所测试的甜味剂中表现出最强的微生物调节潜力。需要进一步的全面临床研究。
