Abstract：
BACKGROUND: The miR-451 has been reported to play an important role in colorectal cancer (CRC) pathogenesis and can be a pivotal diagnosis biomarker of CRC. Given the contradictions in the diagnosis value of the miR-451 in patients with CRC, deciphering the diagnostic/prognostic role of this miRNA in CRC will support the identification of a novel therapeutic target for CRC. Therefore, in the present meta-analysis, we evaluated the diagnostic value of miR-451 in CRC patients.
METHODS: The electronic databases of Embase, PubMed, ISI Web of Science, and Scopus systematically searched for relevant studies. The odds ratio (OR) with a 95% confidence interval (CI) was calculated to evaluate the association between miR-451 family expression and diagnosis of colorectal cancer. The parameters including sensitivity, specificity, and area under the curve (AUC) were obtained. The quality of evidence was evaluated using the Newcastle-Ottava Scale (NOS).
RESULTS: This study involved 510 patients (45% female and 55% male) with CRC. The pooled analysis of the studies showed a significant association between low expression levels of miR-451 in patients with CRC (OR = 7.59; 95% CI 2.39 - 24.07; p = 0.001). The overall sensitivity and specificity were 0.95 (0.61 - 1) and 0.83 (0.43 - 0.99), respectively. The pooled AUC was 0.97 (0.88 - 1; p < 0.006). Results showed if the pre-test probability is 50% for a patient, the post-test probability will be 85%. The indices demonstrated the high potency of miR-451 as a diagnostic biomarker in patients with CRC. No publication bias was observed using the Begg\'s (p=0.85) and Egger\'s tests (p=0.45).
CONCLUSIONS: A strong relationship between the low expression levels of miR-451 and CRC progression was observed. This finding suggests the miR-451 family may be helpful as a potential biomarker for the earlier diagnosis of colorectal cancer.
METHODS: The electronic databases of Embase, PubMed, ISI Web of Science, and Scopus systematically searched for relevant studies. The odds ratio (OR) with a 95% confidence interval (CI) was calculated to evaluate the association between miR-451 family expression and diagnosis of colorectal cancer. The parameters including sensitivity, specificity, and area under the curve (AUC) were obtained. The quality of evidence was evaluated using the Newcastle-Ottava Scale (NOS).
RESULTS: This study involved 510 patients (45% female and 55% male) with CRC. The pooled analysis of the studies showed a significant association between low expression levels of miR-451 in patients with CRC (OR = 7.59; 95% CI 2.39 - 24.07; p = 0.001). The overall sensitivity and specificity were 0.95 (0.61 - 1) and 0.83 (0.43 - 0.99), respectively. The pooled AUC was 0.97 (0.88 - 1; p < 0.006). Results showed if the pre-test probability is 50% for a patient, the post-test probability will be 85%. The indices demonstrated the high potency of miR-451 as a diagnostic biomarker in patients with CRC. No publication bias was observed using the Begg\'s (p=0.85) and Egger\'s tests (p=0.45).
CONCLUSIONS: A strong relationship between the low expression levels of miR-451 and CRC progression was observed. This finding suggests the miR-451 family may be helpful as a potential biomarker for the earlier diagnosis of colorectal cancer.
摘要：
背景：据报道，miR-451在结直肠癌（CRC）的发病机制中起重要作用，并且可以作为CRC的关键诊断生物标志物。鉴于miR-451在CRC患者中的诊断价值存在矛盾,破译该miRNA在CRC中的诊断/预后作用将支持鉴定CRC的新治疗靶标。因此,在目前的荟萃分析中，我们评估了miR-451在CRC患者中的诊断价值.
方法：Embase的电子数据库，PubMed,ISIWebofScience,和Scopus系统地搜索相关研究。计算具有95%置信区间(CI)的比值比(OR)以评估miR-451家族表达与结直肠癌诊断之间的关联。参数包括灵敏度，特异性，获得曲线下面积(AUC)。使用纽卡斯尔-奥塔瓦量表（NOS）评估证据质量。
结果：本研究涉及510例CRC患者（45％为女性，55％为男性）。研究的汇总分析显示，CRC患者中miR-451的低表达水平之间存在显着关联（OR=7.59;95％CI2.39-24.07;p=0.001）。总体敏感性和特异性分别为0.95（0.61-1）和0.83（0.43-0.99），分别。合并的AUC为0.97(0.88-1;p<0.006)。结果显示，如果患者的预测试概率为50%，测试后的概率将是85%。这些指数表明miR-451作为CRC患者的诊断生物标志物具有很高的效力。使用Begg检验（p=0.85）和Egger检验（p=0.45）未观察到发表偏倚。
结论：观察到miR-451的低表达水平与CRC进展之间有很强的关系。这一发现表明miR-451家族可能有助于作为早期诊断结直肠癌的潜在生物标志物。
方法：Embase的电子数据库，PubMed,ISIWebofScience,和Scopus系统地搜索相关研究。计算具有95%置信区间(CI)的比值比(OR)以评估miR-451家族表达与结直肠癌诊断之间的关联。参数包括灵敏度，特异性，获得曲线下面积(AUC)。使用纽卡斯尔-奥塔瓦量表（NOS）评估证据质量。
结果：本研究涉及510例CRC患者（45％为女性，55％为男性）。研究的汇总分析显示，CRC患者中miR-451的低表达水平之间存在显着关联（OR=7.59;95％CI2.39-24.07;p=0.001）。总体敏感性和特异性分别为0.95（0.61-1）和0.83（0.43-0.99），分别。合并的AUC为0.97(0.88-1;p<0.006)。结果显示，如果患者的预测试概率为50%，测试后的概率将是85%。这些指数表明miR-451作为CRC患者的诊断生物标志物具有很高的效力。使用Begg检验（p=0.85）和Egger检验（p=0.45）未观察到发表偏倚。
结论：观察到miR-451的低表达水平与CRC进展之间有很强的关系。这一发现表明miR-451家族可能有助于作为早期诊断结直肠癌的潜在生物标志物。
