Abstract：
Detecting and quantifying amino acids is vital in biochemical analyses, especially for diagnosing metabolic disorders. L-proline, among these amino acids, holds significant relevance for various metabolic disorders in living organisms, particularly in humans. hyperprolinemia arises when ineffective breakdown of L-proline occurs due to enzyme deficiencies, leading to its accumulation in the body and underscoring the need for precise monitoring. To address this challenge, molecular imprinting offers a reliable single-step technique for detecting target molecules like proteins, peptides, amino acids, or ions with high selectivity. Moreover, nanoparticles, with significant surface area-to-volume ratios, enable high-level mass transfer and binding kinetics, making them ideal for nano-scale sensitive applications. In this study, 2-hydroxyethyl methacrylate-based molecularly imprinted nanoparticles were synthesized via mini-emulsion polymerization, combining the advantages of molecular imprinting technique and nanoparticles for the specific recognition of L-proline, and were well-characterized by Scanning Electron Microscopy, zeta-sizer particle size analysis, and Fourier Transform Infrared Spectroscopy. Based on zeta-sizer analysis, the estimated diameters of L-proline-imprinted and non-imprinted nanoparticles (Pro-MIPs and NIPs) were determined to be approximately 27.51 nm and 20.66 nm, respectively. The adsorption of L-proline onto nanoparticles from aqueous solutions was investigated in a batch system, and the maximum L-proline adsorption capacity was determined to be 26.58 mg/g for Pro-MIPs and 4.65 mg/g for and NIPs. The selectivity of Pro-MIPs was assessed using Liquid Chromatography-Tandem Mass Spectrometry, even in human serum and in the presence of competing molecules (L-histidine and L-phenylalanine). Additionally, Pro-MIPs maintained their adsorption capacity through up to 10 adsorption-desorption cycles without significant decrease.
摘要：
检测和定量氨基酸在生化分析中至关重要，特别是用于诊断代谢紊乱。L-脯氨酸,在这些氨基酸中，与生物体中的各种代谢紊乱具有重要的相关性，特别是在人类中。当由于酶缺乏导致L-脯氨酸的无效分解时，就会出现高脯氨酸血症，导致其在体内的积累，并强调需要精确监测。为了应对这一挑战，分子印迹提供了一种可靠的单步技术来检测靶分子，如蛋白质，肽，氨基酸,或具有高选择性的离子。此外，纳米粒子，具有显著的表面积-体积比，实现高水平的传质和结合动力学，使它们成为纳米级敏感应用的理想选择。在这项研究中,通过微乳液聚合合成了甲基丙烯酸2-羟乙酯基分子印迹纳米颗粒,结合分子印迹技术和纳米颗粒的优势，用于L-脯氨酸的特异性识别，并通过扫描电子显微镜得到了很好的表征，zeta-sizer粒度分析，和傅里叶变换红外光谱。基于zeta-sizer分析，L-脯氨酸印迹和非印迹纳米颗粒（Pro-MIPs和NIPs）的估计直径被确定为约27.51nm和20.66nm，分别。在间歇系统中研究了L-脯氨酸在水溶液中纳米颗粒上的吸附，Pro-MIPs的最大L-脯氨酸吸附量为26.58mg/g，NIPs为4.65mg/g。使用液相色谱-串联质谱法评估Pro-MIP的选择性，甚至在人血清中和存在竞争分子(L-组氨酸和L-苯丙氨酸)的情况下。此外,Pro-MIP通过多达10个吸附-解吸循环保持其吸附能力，而没有显着降低。
