Abstract：
In the present study, we investigated the mechanisms associated with the stabilizing effects of whey protein concentrate-80 (WPC80) and L-ascorbyl 6-palmitate (LAP) on folic acid (FA). Multispectral techniques show that WPC80 binds to FA and LAP mainly through hydrophobic interactions, and that energy is transferred from WPC80 to FA and LAP in a nonradiative form (FA/LAP); The combination of FA/LAP resulted in a change in the conformation and secondary structure content of WPC80, an increase in the absolute zeta potential of the system, and a shift in the particle size distribution towards smaller sizes. The compound system exhibits strengthened antioxidant properties and favorable binding properties. Besides, WPC80 improves the storage stability of FA under different conditions. These results demonstrated that the ternary complex formed by FA co-binding with WPC80 and LAP is an effective way to improve the stability against of FA.
摘要：
在本研究中,我们研究了与乳清蛋白浓缩物80（WPC80）和L-抗坏血酸6-棕榈酸酯（LAP）对叶酸（FA）的稳定作用相关的机制。多光谱技术表明，WPC80主要通过疏水相互作用与FA和LAP结合，能量以非辐射形式（FA/LAP）从WPC80转移到FA和LAP；FA/LAP的组合导致WPC80的构象和二级结构含量发生变化，系统的绝对zeta电位增加，以及粒度分布向较小尺寸的偏移。该复合体系表现出增强的抗氧化性能和良好的粘合性能。此外,WPC80提高了FA在不同条件下的储存稳定性。这些结果表明，FA与WPC80和LAP共结合形成的三元复合物是提高FA稳定性的有效途径。
