PDF(Pubmed)
Abstract：
BACKGROUND: Foliage color is considered an important ornamental character of Cymbidium tortisepalum (C. tortisepalum), which significantly improves its horticultural and economic value. However, little is understood on the formation mechanism underlying foliage-color variations.
METHODS: In this study, we applied a multi-omics approach based on transcriptomics and metabolomics, to investigate the biomolecule mechanisms of metabolites changes in C. tortisepalum colour mutation cultivars.
RESULTS: A total of 508 genes were identified as differentially expressed genes (DEGs) between wild and foliage colour mutation C. tortisepalum cultivars based on transcriptomic data. KEGG enrichment of DEGs showed that genes involved in phenylalanine metabolism, phenylpropanoid biosynthesis, flavonoid biosynthesis and brassinosteroid biosynthesis were most significantly enriched. A total of 420 metabolites were identified in C. tortisepalum using UPLC-MS/MS-based approach and 115 metabolites differentially produced by the mutation cultivars were identified. KEGG enrichment indicated that the most metabolites differentially produced by the mutation cultivars were involved in glycerophospholipid metabolism, tryptophan metabolism, isoflavonoid biosynthesis, flavone and flavonol biosynthesis. Integrated analysis of the metabolomic and transcriptomic data showed that there were four significant enrichment pathways between the two cultivars, including phenylalanine metabolism, phenylpropanoid biosynthesis, flavone and flavonol biosynthesis and flavonoid biosynthesis.
CONCLUSIONS: The results of this study revealed the mechanism of metabolites changes in C. tortisepalum foliage colour mutation cultivars, which provides a new reference for breeders to improve the foliage color of C. tortisepalum.
METHODS: In this study, we applied a multi-omics approach based on transcriptomics and metabolomics, to investigate the biomolecule mechanisms of metabolites changes in C. tortisepalum colour mutation cultivars.
RESULTS: A total of 508 genes were identified as differentially expressed genes (DEGs) between wild and foliage colour mutation C. tortisepalum cultivars based on transcriptomic data. KEGG enrichment of DEGs showed that genes involved in phenylalanine metabolism, phenylpropanoid biosynthesis, flavonoid biosynthesis and brassinosteroid biosynthesis were most significantly enriched. A total of 420 metabolites were identified in C. tortisepalum using UPLC-MS/MS-based approach and 115 metabolites differentially produced by the mutation cultivars were identified. KEGG enrichment indicated that the most metabolites differentially produced by the mutation cultivars were involved in glycerophospholipid metabolism, tryptophan metabolism, isoflavonoid biosynthesis, flavone and flavonol biosynthesis. Integrated analysis of the metabolomic and transcriptomic data showed that there were four significant enrichment pathways between the two cultivars, including phenylalanine metabolism, phenylpropanoid biosynthesis, flavone and flavonol biosynthesis and flavonoid biosynthesis.
CONCLUSIONS: The results of this study revealed the mechanism of metabolites changes in C. tortisepalum foliage colour mutation cultivars, which provides a new reference for breeders to improve the foliage color of C. tortisepalum.
摘要：
背景：叶子颜色被认为是大花兰的重要装饰性特征（C.tortisepalum),显着提高了其园艺和经济价值。然而,对叶子颜色变化的形成机制知之甚少。
方法：在本研究中，我们应用了基于转录组学和代谢组学的多组学方法，目的探讨牛背叶草颜色突变品种代谢产物变化的生物分子机制。
结果：基于转录组数据，总共508个基因被鉴定为野生和叶子颜色突变C.tortisepalum品种之间的差异表达基因（DEG）。KEGG富集的DEGs表明，参与苯丙氨酸代谢的基因，苯丙素生物合成，类黄酮生物合成和油菜素类固醇生物合成最显著富集。使用基于UPLC-MS/MS的方法在C.tortisepalum中鉴定了总共420种代谢物，并鉴定了由突变品种差异产生的115种代谢物。KEGG富集表明，突变品种差异产生的大多数代谢产物参与甘油磷脂的代谢。色氨酸代谢,异黄酮生物合成，黄酮和黄酮醇的生物合成。对代谢组和转录组数据的综合分析表明，两个品种之间存在四个显著的富集途径，包括苯丙氨酸代谢，苯丙素生物合成，黄酮和黄酮醇的生物合成和类黄酮的生物合成。
结论：这项研究的结果揭示了黄柏叶色突变品种中代谢物变化的机制，为育种者改善牛角叶色提供了新的参考。
方法：在本研究中，我们应用了基于转录组学和代谢组学的多组学方法，目的探讨牛背叶草颜色突变品种代谢产物变化的生物分子机制。
结果：基于转录组数据，总共508个基因被鉴定为野生和叶子颜色突变C.tortisepalum品种之间的差异表达基因（DEG）。KEGG富集的DEGs表明，参与苯丙氨酸代谢的基因，苯丙素生物合成，类黄酮生物合成和油菜素类固醇生物合成最显著富集。使用基于UPLC-MS/MS的方法在C.tortisepalum中鉴定了总共420种代谢物，并鉴定了由突变品种差异产生的115种代谢物。KEGG富集表明，突变品种差异产生的大多数代谢产物参与甘油磷脂的代谢。色氨酸代谢,异黄酮生物合成，黄酮和黄酮醇的生物合成。对代谢组和转录组数据的综合分析表明，两个品种之间存在四个显著的富集途径，包括苯丙氨酸代谢，苯丙素生物合成，黄酮和黄酮醇的生物合成和类黄酮的生物合成。
结论：这项研究的结果揭示了黄柏叶色突变品种中代谢物变化的机制，为育种者改善牛角叶色提供了新的参考。
