PDF(Pubmed)
Abstract：
Globozoospermia is a rare sperm morphological abnormality characterized by a lack of acrosomes and post-acrosomal sheaths, defects in the cytoskeleton around the nucleus, and separated nuclear membranes. In this case, the study outlines the treatment of a 32-year-old male patient diagnosed with globozoospermia. The couple, facing primary infertility for seven years, had already undergone unsuccessful assisted reproductive technology treatments, such as two intrauterine inseminations and one in vitro fertilization. They opted for intracytoplasmic sperm injection (ICSI) with assisted oocyte activation (AOA) using a calcium (Ca) ionophore. The semen analysis showed globozoospermia, which indicated that ICSI was required for fertilization. Post-fertilization, embryo quality was assessed; three were in cleavage-stage embryos, and two grade 4AA and 3AA blastocysts and the rest were arrested at 2 pronuclear (2PN) stages, revealing successful embryo development. This case report implies that using AOA with Ca ionophores enhanced the fertilization outcomes and could be a helpful intervention strategy for patients with globozoospermia.
摘要：
球精子症是一种罕见的精子形态异常，其特征是缺乏顶体和顶体后鞘。细胞核周围细胞骨架的缺陷，和分离的核膜。在这种情况下,该研究概述了一名32岁男性患者的治疗方法。这对夫妇,面对原发性不孕症七年，已经接受了不成功的辅助生殖技术治疗，如两次宫腔内授精和一次体外受精。他们选择了使用钙（Ca）离子载体辅助卵母细胞激活（AOA）的胞浆内单精子注射（ICSI）。精液分析显示球精子症，这表明受精需要ICSI。受精后,评估了胚胎质量；三个在卵裂期胚胎中，两个4AA和3AA级囊胚和其余的在2个原核（2PN）阶段被逮捕，揭示成功的胚胎发育。此病例报告表明，使用AOA和Ca离子载体可增强受精结果，对于球精子症患者可能是一种有用的干预策略。
