PDF(Pubmed)
Abstract：
Ophthalmic and many systemic diseases may damage the eyes, resulting in changes in the composition and content of biomolecules in ocular biofluids such as aqueous humor and tear. Therefore, the biomolecules in biofluids are potential biomarkers to reveal pathological processes and diagnose diseases. Raman spectroscopy is a non-invasive, label-free, and cost-effective technique to provide chemical bond information of biomolecules and shows great potential in the detection of ocular biofluids. This review demonstrates the applications of Raman spectroscopy technology in detecting biochemical components in aqueous humor and tear, then summarizes the current problems encountered for clinical applications of Raman spectroscopy and looks forward to possible approaches to overcome technical bottlenecks. This work may provide a reference for wider applications of Raman spectroscopy in biofluid detection and inspire new ideas for the diagnosis of diseases using ocular biofluids.
摘要：
眼科和许多全身性疾病可能会损害眼睛，导致眼生物流体如房水和泪液中生物分子的组成和含量的变化。因此,生物流体中的生物分子是揭示病理过程和诊断疾病的潜在生物标志物。拉曼光谱是一种非侵入性、无标签，和经济有效的技术来提供生物分子的化学键信息，并在检测眼部生物流体方面显示出巨大的潜力。本文综述了拉曼光谱技术在房水和泪液生化成分检测中的应用。然后总结了拉曼光谱在临床应用中遇到的问题，并展望了克服技术瓶颈的可能方法。这项工作可以为拉曼光谱在生物流体检测中的更广泛的应用提供参考，并为使用眼部生物流体诊断疾病提供新的思路。
