PDF(Pubmed)
Abstract：
Having been successfully bred in semi-intensive and intensive aquaculture systems, oval squids of the Sepioteuthis lessoniana species complex are emerging as promising candidates for research and industry. Nevertheless, information about pathogens and diseases that may affect squid aquaculture remains sparse. In this study, we identify new parasitic copepod species that causes squid mortality and decreases squid hatching rates, and we also offer a solution to eliminate the pathogen during incubation of squid eggs. The newly discovered copepod Ikanecator primus gen. et sp. nov. was identified on oval squid eggs for the first time using both morphological and molecular diagnostic markers. In the genomes of the copepod and associated microbiome, we identified multiple genes for enzymes involved in cephalopod eggshell degradation in genomes of the copepod and associated microbiome. Furthermore, we conducted experiments to assess efficacy of peracetic acid in inhibiting the I. primus gen. et sp. nov. both in vitro and in vivo using immersion treatment. We established that a 2-min exposure to a concentration of 250 μl/L of peracetic acid containing product (PAA-product; 35 mg/L PAA and 15 mg/L H2O2) inhibited the development of nauplii in vitro. All parasites exposed to a concentration of 500 μl/L of PAA-product (70 mg/L PAA and 30 mg/L H2O2) were eliminated within two minutes. On top of this, the immersion treatment with 500 μl/L of PAA-product (70 mg/L PAA and 30 mg/L H2O2) improved survival of squid embryos and increased size of squid hatchlings compared with control and the immersion treatment with 125 μl/L of PAA-product (17.5 mg/L PAA and 7.5 mg/L H2O2) and the immersion treatment with 250 μl/L of PAA-product (35 mg/L PAA and 15 mg/L H2O2). These findings suggest that PAA holds a great potential as inhibitor and controller of parasitic copepod infections and for overall health management in cephalopod culture.
摘要：
在半集约化和集约化水产养殖系统中成功繁殖，Sepioteuthislessoniana物种复合体的椭圆形鱿鱼正在成为有希望的研究和工业候选人。然而，关于可能影响鱿鱼水产养殖的病原体和疾病的信息仍然很少。在这项研究中,我们发现了新的寄生co足类物种，它们会导致鱿鱼死亡并降低鱿鱼孵化率，我们还提供了在鱿鱼卵孵化过程中消除病原体的解决方案。新发现的co足动物Ikanecatorprimusgen。等sp.11月。首次使用形态学和分子诊断标记在椭圆形鱿鱼卵上鉴定。在co足类和相关微生物组的基因组中，我们确定了在co足类和相关微生物组的基因组中参与头足类蛋壳降解的酶的多个基因。此外，我们进行了实验以评估过乙酸抑制I.primusgen的功效。等sp.11月。在体外和体内使用浸泡治疗。我们确定，暴露于浓度为250μl/L的过乙酸产品（PAA产品；35mg/LPAA和15mg/LH2O2）2分钟会在体外抑制无节幼体的发展。暴露于浓度为500μl/L的PAA-产物(70mg/LPAA和30mg/LH2O2)的所有寄生虫在两分钟内被消除。最重要的是，与对照相比，用500μl/LPAA产品（70mg/LPAA和30mg/LH2O2）的浸泡处理提高了鱿鱼胚胎的存活率，并增加了鱿鱼孵出的大小，用125μl/LPAA产品（17.5mg/LPAA和7.5mg/LH2O2）和250μl/LPAA产品（35mg/LPAA和15mgH2O2）的浸泡处理。这些发现表明，PAA具有巨大的潜力，可作为寄生co足类动物感染的抑制剂和控制器，并可用于头足类养殖的整体健康管理。
