PDF(Pubmed)
Abstract：
Epidural spinal cord stimulation (SCS) is indicated for the treatment of intractable pain and is widely used in clinical practice. In previous basic research, the therapeutic effects of SCS have been demonstrated for epileptic seizure. However, the mechanism has not yet been elucidated. In this study, we investigated the therapeutic effect of SCS and the influence of epileptic seizure. First, SCS in the cervical spine was performed. The rats were divided into four groups: control group and treatment groups with SCS conducted at 2, 50, and 300 Hz frequency. Two days later, convulsions were induced by the intraperitoneal administration of kainic acid, followed by video monitoring to assess seizures. We also evaluated glial cells in the hippocampus by fluorescent immunostaining, electroencephalogram measurements, and inflammatory cytokines such as C-C motif chemokine ligand 2 (CCL2) by quantitative real-time polymerase chain reaction. Seizure frequency and the number of glial cells were significantly lower in the 300 Hz group than in the control group. SCS at 300 Hz decreased gene expression level of CCL2, which induces monocyte migration. SCS has anti-seizure effects by inhibiting CCL2-mediated cascades. The suppression of CCL2 and glial cells may be associated with the suppression of epileptic seizure.
摘要：
硬膜外脊髓刺激（SCS）用于治疗顽固性疼痛，并在临床实践中广泛使用。在以往的基础研究中,已经证明了SCS对癫痫发作的治疗作用。然而,机制尚未阐明。在这项研究中,我们调查了SCS的治疗效果和癫痫发作的影响。首先,在颈椎中进行SCS。将大鼠分为四组：对照组和以2、50和300Hz频率进行SCS的治疗组。两天后,通过腹膜内施用海藻酸来引起惊厥，随后进行视频监控以评估癫痫发作。我们还通过荧光免疫染色评估了海马中的神经胶质细胞，脑电图测量，和炎性细胞因子如C-C基序趋化因子配体2(CCL2)通过定量实时聚合酶链反应。300Hz组癫痫发作频率和胶质细胞数量明显低于对照组。300Hz的SCS降低了CCL2的基因表达水平，从而诱导了单核细胞的迁移。SCS通过抑制CCL2介导的级联反应而具有抗癫痫作用。抑制CCL2和神经胶质细胞可能与抑制癫痫发作有关。
