Abstract：
Quantifying signals substantially increases the efficiency of fluorescence in situ hybridization (FISH). Quantitative FISH analysis or QFISHing may be useful for differentiation between chromosome loss and chromosomal associations, detection of amplification of chromosomal loci, and/or quantification of chromosomal heteromorphisms (chromosomal DNAs). The latter is applicable to uncovering the parental origin of chromosomes, which is an important FISH application in genome research. In summary, one may acknowledge that QFISHing has a variety of applications in cancer chromosome research. Accordingly, a protocol for this technique is certainly required. Here, QFISHing protocol is described step-by-step.
摘要：
定量信号显著提高了荧光原位杂交(FISH)的效率。定量FISH分析或QFISHing可能有助于区分染色体丢失和染色体关联，染色体位点扩增检测，和/或染色体异态(染色体DNA)的定量。后者适用于揭示染色体的亲本起源，这是FISH在基因组研究中的重要应用。总之,人们可能承认QFISHing在癌症染色体研究中具有多种应用。因此，这种技术的协议当然是必需的。这里,QFISHing协议是逐步描述的。
