%0 Journal Article
%T Quantitative FISHing: Implications for Chromosomal Analysis.
%A Vorsanova SG
%A Yurov YB
%A Iourov IY
%J Methods Mol Biol
%V 2825
%N 0
%D 2024
%M 38913313
暂无%R 10.1007/978-1-0716-3946-7_13
%X Quantifying signals substantially increases the efficiency of fluorescence in situ hybridization (FISH). Quantitative FISH analysis or QFISHing may be useful for differentiation between chromosome loss and chromosomal associations, detection of amplification of chromosomal loci, and/or quantification of chromosomal heteromorphisms (chromosomal DNAs). The latter is applicable to uncovering the parental origin of chromosomes, which is an important FISH application in genome research. In summary, one may acknowledge that QFISHing has a variety of applications in cancer chromosome research. Accordingly, a protocol for this technique is certainly required. Here, QFISHing protocol is described step-by-step.