Abstract：
OBJECTIVE: This study aims to analyze the prevalence of Candida spp. colonization in oral leukoplakia and oral lichen planus lesions, verify the influence of systemic and local factors, besides identify and determine the in vitro antifungal susceptibility profile of Candida species.
METHODS: Samples were collected by swabbing from oral lesions and healthy mucosa and cultured on Sabouraud Dextrose and CHROMagar® Candida plates. Species identification was confirmed with MALDI-TOF MS analysis.
RESULTS: Candida spp. was found in 36.8% of cases of oral leukoplakia and 18.2% of cases of oral lichen planus. Candida albicans was the only species found in oral lichen planus lesions (n = 2, 100%) and the most prevalent in oral leukoplakia (n = 5, 76.4%). Among the non-albicans Candida species found in oral leukoplakia were C. parapsilosis (n = 2, 25.5%) and C. tropicalis (n = 1, 14.1%). Candida isolates were susceptible to all antifungals tested.
CONCLUSIONS: C. albicans was the most commonly found species in the studied lesions. No correlation was found between systemic and local factors with positive cases of oral lichen planus. However, smoking and alcohol consumption may be associated with positive cases of oral leukoplakia, especially the non-homogeneous clinical form. In addition, there is a possible predisposition to associated Candida colonization in cases of epithelial dysplasia found in oral leukoplakia. The antifungal medications tested showed excellent efficacy against isolates.
METHODS: Samples were collected by swabbing from oral lesions and healthy mucosa and cultured on Sabouraud Dextrose and CHROMagar® Candida plates. Species identification was confirmed with MALDI-TOF MS analysis.
RESULTS: Candida spp. was found in 36.8% of cases of oral leukoplakia and 18.2% of cases of oral lichen planus. Candida albicans was the only species found in oral lichen planus lesions (n = 2, 100%) and the most prevalent in oral leukoplakia (n = 5, 76.4%). Among the non-albicans Candida species found in oral leukoplakia were C. parapsilosis (n = 2, 25.5%) and C. tropicalis (n = 1, 14.1%). Candida isolates were susceptible to all antifungals tested.
CONCLUSIONS: C. albicans was the most commonly found species in the studied lesions. No correlation was found between systemic and local factors with positive cases of oral lichen planus. However, smoking and alcohol consumption may be associated with positive cases of oral leukoplakia, especially the non-homogeneous clinical form. In addition, there is a possible predisposition to associated Candida colonization in cases of epithelial dysplasia found in oral leukoplakia. The antifungal medications tested showed excellent efficacy against isolates.
摘要：
目的：本研究旨在分析念珠菌的患病率。口腔白斑和口腔扁平苔藓病变的定植，验证系统和局部因素的影响，除了鉴定和确定念珠菌的体外抗真菌药敏谱。
方法：通过擦拭从口腔病变和健康粘膜收集样品，并在Sabouraud葡萄糖和CHROMagar®念珠菌平板上培养。用MALDI-TOFMS分析确认物种鉴定。
结果：念珠菌属。36.8%的口腔白斑和18.2%的口腔扁平苔藓。白色念珠菌是在口腔扁平苔藓病变中发现的唯一物种（n=2，100％），在口腔白斑中最普遍（n=5，76.4％）。在口腔白斑中发现的非白色念珠菌中，有梭菌（n=2，25.5％）和热带念珠菌（n=1，14.1％）。念珠菌分离物对所有测试的抗真菌药物敏感。
结论：C.白色念珠菌是所研究病变中最常见的物种。在全身和局部因素与口腔扁平苔藓阳性病例之间未发现相关性。然而,吸烟和饮酒可能与口腔白斑阳性病例有关，尤其是非均匀的临床形式。此外,在口腔白斑中发现的上皮异型增生病例中，可能存在相关念珠菌定植的倾向。测试的抗真菌药物对分离物显示出优异的功效。
方法：通过擦拭从口腔病变和健康粘膜收集样品，并在Sabouraud葡萄糖和CHROMagar®念珠菌平板上培养。用MALDI-TOFMS分析确认物种鉴定。
结果：念珠菌属。36.8%的口腔白斑和18.2%的口腔扁平苔藓。白色念珠菌是在口腔扁平苔藓病变中发现的唯一物种（n=2，100％），在口腔白斑中最普遍（n=5，76.4％）。在口腔白斑中发现的非白色念珠菌中，有梭菌（n=2，25.5％）和热带念珠菌（n=1，14.1％）。念珠菌分离物对所有测试的抗真菌药物敏感。
结论：C.白色念珠菌是所研究病变中最常见的物种。在全身和局部因素与口腔扁平苔藓阳性病例之间未发现相关性。然而,吸烟和饮酒可能与口腔白斑阳性病例有关，尤其是非均匀的临床形式。此外,在口腔白斑中发现的上皮异型增生病例中，可能存在相关念珠菌定植的倾向。测试的抗真菌药物对分离物显示出优异的功效。
